Amytracker are small fluorescent molecules for detection of protein aggregates suitable for use in fibrillation assays, fixed or fresh tissue sections and cells as well as systemic injection in vivo. They are exceptionally photo- and thermostable and allow for easy handling in any application. Amytracker work in a wide range of salt and pH conditions. When the pH is altered during the experiment, pH controls should be included.

Amytracker can be used with fluorescence plate readers, fluorescence microscopes and confocal laser scanning microscopes, fluorescence life time imaging (FLIM), fluorescence cytometry (FACS), Total internal reflection fluorescence (TIRF) microscopy and Multiphoton microscopy

Five Amytracker variants are available specified by their peak emission wavelength. All Amytracker variants are designed to bind to the Congo red binding pocket on the amyloid fibril. A minimum of eight in-register parallel-β-strands are required for binding. The Amytracker variants differ in affinity and spectral properties. As Amytracker are structural markers, you can achieve reliable fluorescent labeling of amyloids derived from a variety of amyloidogenic proteins or peptides from different species.

You can choose between four different formulations. 

• Aqueous: 1 mg/ml solution in ultrapure water. The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
• DMSO: 1 mg/ml solution in DMSO to prevent solvent evaporation. The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations
• Solid: 1 mg solid lyophilised in a sterile injection bottle. We recommend dilution to 4 mg/ml in physiological saline followed by intravenous injection with a total dose of 5 mg/KG
• Drop&Shine: 5 ml ready-to-use product in mounting medium. Ideal for use in tissue sections. Add a some Drop&Shine and mount your slide to detect amyloids within minutes.

Store your Amytracker product in the fridge and use the opened container within 12 months. Amytracker is for research use only and is not for resale.