A repeat expansion in the C9orf72 gene is the most frequent cause of ALS and FTD. Researchers from Max Planck Institute of Biochemistry and University Medical Center Gröningen revealed the molecular mechanism behind Dipeptide Repeat Proteins (DRP) cytotoxicity in their publication in the journal eLife. They used Amytracker 680 to localize and characterize both cytoplasmic and nuclear DRP aggregates.

Image:🔬HEK293 cells transfected with the indicated constructs and labeled with Amytracker 680. (AmyT in red, Poly-GA DPRs in green, nuclear membrane indicated by white dashed lines). Frottin et al. (Fig. 1D) (CC BY 4.0)

Our products are featured in over 65 publications in various high-impact journals, we’re proud to share our contributions to advancing scientific knowledge! From groundbreaking discoveries to innovative technologies worldwide.

Curious to learn more? Reach out to us today!

Yesterday, our COO Smilla connected with lots of engaged students at the Mingle Event for the KI Careers Internship Program, showcasing the exciting internship opportunities at Ebba Biotech. 👨‍🔬

Today, we honor, celebrate, and appreciate the incredible women making a difference around the world.🌎💖

Did you know that Ebba Biotech was founded by a visionary woman - Prof. Agneta Richter-Dahlfors? 🤔 But there’s more! We take immense pride in being a company where exceptional women lead the team 👯‍♀️ and run the office 👭 in order to innovate and push Ebba Biotech forward. 💪🌟

Finally, the news is out! Today marks the launch of our eagerly awaited updated product catalog, featuring three new formulations for all product families and variants: 🌟 DMSO, Drop&Shine, and Solid.🚀

But that’s not all! Remember to explore our original formulation (ultrapure water) under a new name - Aqueous - as well as our top-notch testing service. 👨‍🔬

During the past month, we have enjoyed having Alejandro as our Guest Ph.D. student from the KI Careers Service program.👨‍🔬 He has been able to venture into the world of Life Science start-ups by getting first-hand experience in sales and marketing alongside active product development work in the lab.

It has been a genuine pleasure to get to know you and we look forward to following your career moving forward. 🚀 Thank you, Ale, for your valuable contributions, and we hope our paths cross again in the future. 🌟

Small protein aggregates (below 450 nm) are highly toxic as they are able to penetrate the cell membrane. Their small size makes these aggregates especially hard to study. 💡 With Amytracker, however, you can finally see them! Michael J. Morten and colleagues came up with a new approach that involves using Amytracker 630 and super-resolution microscopy to resolve aggregates involved in neurodegenerative disorders. In their experiments, Amytracker 630 performed much better than conventional staining methods, such as Thioflavin! 🏅

"We show that Amytracker 630 (AT630), a commercial aggregate-activated fluorophore, has outstanding photophysical properties that enable super-resolution imaging of α-synuclein, tau, and amyloid-β aggregates, achieving ∼4 nm precision"

🔬Image: Cell-penetrating aggregate species in HEK cells (Live super-resolution imaging by SMLM.) AmyT 630 (red), Proteasome foci (green). Typical foci-aggregate colocalizations are shown in the zoomed-in images. (white scale bars: 2 μm l and 200 nm), Morten et al. (Fig. 3C) (CC BY 4)

🇸🇪 Today, the entire team was invited to Academic Work to celebrate Shrove Tuesday - or as we call it here in Sweden - Semmeldagen!☕️

Embracing the fika-culture to its fullest by indulging in a cardamom-infused bun filled with a sweet almond paste and whipped cream🍰

Thanks to Malin Kristoffersson for the invitation.🙌

At Ebba Biotech, we believe in more than just providing biotech solutions - we are all about building lasting connections and ensuring customer satisfaction!🌟

🤝 Our team is the heart of Ebba Biotech, driven by a shared commitment to delivering not only fantastic products but also providing exceptional customer support. Every member will go the extra mile to understand and troubleshoot customer issues alongside our users to ensure seamless integration of the optotracer products in labs worldwide.🔬🌈

Reach out and chat with our team members to see how you can get started with optotracers in your lab🤩

What causes the accumulation of toxic amyloid aggregates? An article by Rimal et al. published in the journal Acta Neuropathologica Communications might have found the answer to this question. Their work shows that a defective ribosome quality control (RQC) causes the C-terminal fragment (APP.C99) of the Amyloid precursor protein (APP) to accumulate. This might be one of the earliest pathogenic events of amyloid aggregation. Labeling such aggregates in HeLa cells with Amytracker 680 shows that they have amyloid-like properties.

Image:🔬 Ribosome stall-induced aberrant APP.C99 seeds amyloid ß-42 aggregation in HeLa cells after addition of Aß42 AT tails. Fluorescent microscope images showing aggregation of Aß42 as detected with the aggregation-specific dye Amytracker (in blue). Rimal et al. Fig. S6D (CC-BY-4.0).

🌟 Introducing our newest recruit Alejandro Lastra Romero! 🌟

We are excited to have Alejandro as our Guest PhD student for the entirety of February! 🎉🤝 As a Guest PhD student, Alejandro will be learning about the day-to-day operations of a start-up like Ebba Biotech by sampling all the various roles within our team. 🚀

👨‍🔬 About Alejandro

Alejandro is a biochemist in the last year of his PhD studies at Karolinska Institutet. He originally comes from Sevilla (Spain) and arrived in Stockholm 5 years ago. His background is mainly neuro oncology, since he focuses his research on understanding brain injury produced by radiotherapy, which is translated into cognitive problems afterwards.

🎾 He enjoys playing and watching tennis
🌱 Good gardener skills - he managed to grow tomatoes in Sweden
🍝 Pasta bolognese is his way of life

📸✨ Behind-the-Scenes of our Ebba Biotech Photoshoot!

Last Friday, the incredible Juliana Wolf Garcindo once again worked her magic to capture our company’s essence on camera.

🌟 Catch a sneak peek and meet our stunning team. Stay tuned for more glimpses of our behind-the-scenes moments!

🚀 Welcome 2024 with Ebba Biotech! 🌟

Happy New Year to our incredible community, both to our new and longstanding followers! 😊 As we step into 2024, why not kickstart by rediscovering our diverse product range? Explore the possibilities of enhancing your research with our cutting-edge tools - Amytracker, EbbaBiolight, and Carbotrace! 🧠🦠🌿

💡Wondering how to elevate your research? Check out our quick guide highlighting the opportunities our products can offer you today.

Get in touch and start your journey of discovery.🚀✨

We have recognized that optotracing is a novel concept that requires introduction and can sometimes be challenging. We strive towards making things as easy as possible for you. Therefore, we offer packages for "Microscopic Analysis" and "Spectroscopic Analysis" of your samples. We will provide you with protocols for labelling and data analysis as well as some professionally edited images to visualise your samples in a completely new light!

This past year, our fantastic colleagues Hugo & John moved on to new exciting adventures. We want to extend our heartfelt gratitude for their contributions to the success of Ebba Biotech. While it was not easy to fill their shoes, we are happy to announce that Emilie and Davide have now joined our team! Their arrival brings a wave of fresh perspectives and expertise that we eagerly embrace.

Emilie has recently graduated from Stockholm University with a MSc in Biology and a specialisation in plant biology and aquatic microbiology. While she is now just getting started, she will be our key contact person and technical support for the EbbaBiolight and Carbotrace product families.

Davide is a neuroscience specialist who recently obtained his PhD from Karolinska Institutet. With his extensive experience in Neuroscience research and Fluorescense imaging, he'll be our key contact for inquiries related to Amytracker. Naturally, he'll put his creative energy towards launching new products that will serve your projects!

As we near the end of 2023, the Ebba Biotech team extends heartfelt gratitude to our followers! 🌟

This festive season, we reflect on the milestones we have achieved and the connections we have made with both our followers and customers worldwide. It has been an incredible journey, and now we are gearing up to welcome 2024 with our goals and targets set!✨

Get ready for a year filled with new events and exciting announcements as we continue to bring innovative developments to the world of biotech 🚀

From all of us at Ebba Biotech, here is to a joyful holiday season and a prosperous New Year! 🤩

Thank you for being part of our community - we can not wait to kickstart the new year with you 🎇

Recognize Smilla from our LinkedIn, Zoom, emails or Conferences? 🤔

How much do you actually know about her and what she does at Ebba Biotech? We had a chat with Smilla and got her to tell us all about her journey with us.

Here are a few highlights from Smilla’s time with us✨

🌈 Experiencing and contributing to the growth of Ebba Biotech while also growing professionally from a Research Assistant to COO.
🌍 Showcasing the Ebba Biotech products internationally at conferences and meeting all our customers!
👥 Recruiting! I have had the chance to add new members to the Ebba Biotech team. Super challenging - and super rewarding!

Thank you Smilla for being a part of our Ebba Biotech Team!

🏅Today the Ebba Biotech team had the privilege of attending the captivating lecture presented by Nobel Laureates Katalin Karikó and Drew Weissman - winners of The Nobel Prize in Physiology or Medicine 2023. Their groundbreaking contributions to mRNA research are paving the way for revolutionary advancements in modern medicine.

🚀Inspired and motivated, we are now committed more than ever to push the boundaries of innovation and contributing to the future of life science and healthcare.🌟

Molecular protein chaperons are known for their role in preventing protein misfolding and aggregation. However, a paper published in the journal Acta Neuropathologica showed that there might be more to it. In the paper, Rachel E. Lackie and colleagues found that STI1 (a co-chaperon) directly interacts with the α-synuclein and enhances aggregation. They found that in Parkinson’s disorder conditions the expression level of STI1 and Hsp90 pair (chaperon and co-chaperon) is elevated and causes increased aggregation of α-synuclein and its S129 phosphorylation (psyn129).

Image: 🔬Characterization of WT human α-synuclein PFFs (Performed filaments) and pathology induced in M83+/- mice. pSyn129 (red) and Amytracker (green). Top row - M83+/- mice brain section without PFF, bottom row with PFF. Lackie at al. (Fig. Sup S2) (CC-BY-4.0)

Exciting news! - Our Founder Agneta Richter-Dahlfors will attend Forest Innovation Summit 🌲 in San Francisco!

Agneta will represent her company Richter Life Science with a booth at the conference. In her start-up pitch after lunch, Agneta will tell you all about how Richter Life Science takes the guesswork out of fibre analytics! 🚀

Do you want to learn more or register? Visit the website here

🌟 Exciting Announcement!🌟

Join us in welcoming our newest member Davide Maria Trevisan to the Ebba Biotech family! We’re thrilled to have Davide on board as our Product Manager, bringing scientific expertise and a genuine excitement to push Ebba Biotech forward in the Life Science industry.

👨‍🔬 About Davide 👨‍🔬

Some of you have already seen my face here, as I initially joined Ebba Biotech as a guest PhD student back in 2022. I am now back full-time! My name is Davide. I am originally from Italy, but I have been in Sweden long enough to call it home. I recently defended my PhD thesis (so really I am Dr. Davide 🎓😉) and my main expertise is in molecular biology, but I have a Neuroscience background and quite a bit of microscopy experience.

🦖 I never got over my dinosaur phase
🥘 I spend most of my free time cooking
🌲 And what’s left wandering the forest

Thank you Davide for becoming a part of our team!

🔬Did you know that this week is the #BIOFILMWEEK 🧫✨

It’s a fantastic opportunity to shed light on the pivotal role and impact of biofilms! Here at Ebba Biotech, we are thrilled to be part of an industry that is driving innovation to understand and visualise biofilms and their components. A massive thank you to the National Biofilms Innovation Centre for spearheading this initiative.

Thanks to a strong visual impact, the versatile EbbaBiolight optotracer molecule family can help crack the secrets hidden within biofilms. In the image you can see a Salmonella colony growing on EbbaBiolight 680 supplemented LB agar. EbbaBiolight becomes fluorescent when binding to to the bacterial amyloid curli produced by the GFP-tagged Salmonella (green) colony.

How does uropathogenic Escherichia coli establish itself in the hostile environment of the urinary tract to cause infection?

A recent article published in FEMS Microbes by Haris Antypas and colleagues from the Richter-Dahlfors Laboratory has now shed light on this. Their advanced organ-on-chip technology helped reveal the mechanism of adhesion and colony formation under shear stress conditions in the urinary tract. Check out the image below to see how EbbaBiolight was able to show the presence of biofilm in growing microcolonies

Image: 🔬ECM detection using fluorescent microscopy (E. coli biofilm assay at 4 days) (A) with EbbaBiolight 680 (red) and (B) without EbbaBiolight 680. Antypas et al., Fig. 6AB (CC BY 4.0)

Earlier this year, we took a significant step forward by expanding our product series to include DMSO as a solvent for some of Ebba Biotech products, alongside our trusted ultrapure water for dissolving our molecules. Wondering why? Let us fill you in!

💡 Expanding Horizons: With DMSO, we are expanding our application areas to include organic solvents. This minimises any limits on researchers wanting to test different solutions.
🌡 Extended Shelf Life: Our shelf life is improving! As is common with ultrapure water in small volumes - there is always a risk for evaporation. With DMSO we eliminate this risk ensuring maximum quality for a longer period of time.

Do you have any questions about which solvent is right for you, or curious to learn more? Feel free to reach out to us here. We are there to support your research needs! 🔬🧪💼

How can protein aggregation play a role in inherited disorders such as Phenylketonuria (commonly known as PKU)? Oscar Aubi and colleagues published their findings in the journal Nature Communications where they showed a link between aggregation of a mutant phenylalanine hydroxylase (Pah) variant (R261Q) and the disease Phenylketonuria. Amytracker was used to study the aggregation properties of Pah R261Q.

Image: 🔬 Immunofluorescence of wt Pah and Pah-R261Q detection in hepatic tissue. DAPI (blue) and Pah (green). Aubi et al. Fig 4, (CC BY 4.0)