Amytracker for systemic injection

Amytracker can be used for intravenous- or intraperitoneal injection in small animals to label protein aggregates in vivo. It will readily cross the blood brain barrier and can be imaged by intra-vital microscopy or after removing the tissue and preparation of microscope slides. For systemic injection, we recommend to use our Amytracker - Solid formulation that comes in sterile injection bottles.

Solutions and Reagents:

• Amytracker - Solid 1mg
• Physiological saline
• Injection syringes and needles

Assay Procedure:

• Add 250 µl physiological saline to the Amytracker - 1mg Solid by injecting it directly through the rubber stopper. Dissolve all powder residues by shaking or vortexing the bottle.
• Inject a total dose of 5mg/KG of Amytracker intravenously or intraperitoneally.
• You can expect to see staining of cerebral plaques 24-h up to 1 week after injection.

Readout

• For multiphoton microscopty, all Amytracker variants can be excited with a tuneable laser at 800 nm. See the table below for excitation and emission properties of all Amytracker variants with standard microscopy methods.