EbbaBiolight
EbbaBiolight are optotracers for detection of bacteria & components of bacterial biofilm.
EbbaBiolight is available in five variants which label extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight variants have been used as follows: EbbaBiolight 480 labels extracellular matrix produced by P. aeruginosa but not C. albicans in a mircofluidic device during mono- and co-culture. EbbaBiolight 630 and EbbaBiolight 680 label peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. EbbaBiolight 680 labels curli in the extracellular matrix produced by Salmonella as well as β-glucans and chitins in yeast- and hypal forms of C. albicans. It has been used to track pellicle formation in B. cenocepacia and label extracellular matrix components in Pseudomonas and E. coli.
All EbbaBiolight variants are exceptionally photostable and fluorogenic. When bound to a target, the optotracers can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. All EbbaBiolight variants are intended for use in live-cultures. Fixation can lead to unspecific labelling of bacterial cells, regardless of them gram status. If fixation needs to be performed, we recommend to apply fixation after labelling.
EbbaBiolight variant work in a wide range of salt and pH conditions. When the pH is altered during the experiment, pH controls should be included. EbbaBiolight can be used with fluorescence plate readers, fluorescence microscopes and confocal laser scanning microscopes, fluorescence life time imaging, fluorescence cytometry, Total internal reflection fluorescence (TIRF) microscopy and Multiphoton microscopy.
Store your EbbaBiolight product in the fridge and use the opened container within 12 months. EbbaBiolight is for research use only and is not for resale.
EbbaBiolight Mix&Try is our recommended option for starting out with using EbbaBiolight. It contains 10 µL of each variant. Testing each variant in the will allow you to determine which one of our optotracers is best suited for your experiment.
All EbbaBiolight variants label extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, the optotracers have been used as follows: EbbaBiolight 480 labeling extracellular matrix produced by P. aeruginosa but not C. albicans in a mircofluidic device during mono- and co-culture. EbbaBiolight 630 labeling peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. EbbaBiolight 680 labeling curli in the extracellular matrix produced by Salmonella as well as β-glucans, chitin and intracellular amyloid aggregates in yeast- and hypal forms of C. albicans. Furthermore, it has been used to track pellicle biofilm formation in B. cenocepacia. Contact us to learn more about EbbaBiolight applications.
All EbbaBiolight variants are exceptionally photostable and fluorogenic. When bound to a target, the optotracers can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
EbbaBiolight 480 | 420 nm | 480 nm | DAPI |
EbbaBiolight 520 | 460 nm | 520 nm | FITC, GFP |
EbbaBiolight 540 | 480 nm | 540 nm | FITC, GFP, YFP |
EbbaBiolight 630 | 520 nm | 630 nm | PI, Cy3, TxRed, mCherry, Cy3.5 |
EbbaBiolight 680 | 530 nm | 680 nm | PI, mCherry, Cy3.5 |
EbbaBiolight 680 is our red optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 680 labels curli in the extracellular matrix produced by Salmonella as well as β-glucans and chitins in yeast- and hyphal forms of C. albicans. Furthermore, it has been used to track pellicle biofilm formation by B. cenocepacia. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 680 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 680 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
EbbaBiolight 680 | 530 nm | 680 nm | PI, mCherry, Cy3.5 |
EbbaBiolight 680 is available in four different formulations (See volumes and prices in the drop-down list below):
- Aqueous: 1 mg/ml solution in ultrapure water. The product should be diluted 1:1000 before use. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
- DMSO: 1 mg/ml solution in DMSO to prevent solvent evaporation. The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations.
- Solid: 1 mg solid lyophilised in a sterile injection bottle. We recommend dilution to 4 mg/ml in physiological saline followed by intravenous injection with a total dose of 5 mg/KG.
- Drop&Shine: 5 ml ready-to-use product in mounting medium. Ideal for use in tissue sections. Add a some Drop&Shine and mount your slide to detect biofilms within minutes.
EbbaBiolight 630 is our orange optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 630 labels peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 630 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 630 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
EbbaBiolight 630 | 520 nm | 630 nm | PI, Cy3, TxRed, mCherry, Cy3.5 |
EbbaBiolight 630 is available as a 1 mg/ml solution in volumes ranging from 10 - 200 µL (see volumes and prices in the list below). The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
EbbaBiolight 540 is our yellow optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 540 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 540 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
EbbaBiolight 540 | 480 nm | 540 nm | FITC, GFP, YFP |
EbbaBiolight 540 is available as a 1 mg/ml solution in volumes ranging from 10 - 200 µL (see volumes and prices in the list below). The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
EbbaBiolight 520 is our green optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 520 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 520 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
EbbaBiolight 520 | 460 nm | 520 nm | FITC, GFP |
EbbaBiolight 520 is available as a 1 mg/ml solution in volumes ranging from 10 - 200 µL (see volumes and prices in the list below). The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
EbbaBiolight 480 is our blue optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 480 has been used to label extracellular matrix produced by P. aeruginosa but not C. albicans in a mircofluidic device during mono- and co-culture. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 480 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 680 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
EbbaBiolight 480 | 420 nm | 480 nm | DAPI |
EbbaBiolight 480 is available as a 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (see volumes and prices in the list below). The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
Monitoring curli production in liquid culture using EbbaBiolight
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Monitoring curli in bacterial biofilms forming on semi-solid agar
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Labeling of surface biofilm using EbbaBiolight
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The role of polyP in biofilm formation of hypervirulent Klebsiella pneumoniae
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Real-time insights into curli and cellulose dynamics in UPEC
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Use of EbbaBiolight in the development of a new root canal sterilisation tool
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How EbbaBiolight helps researchers to develop biofilm models
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Biofilm formation of UPEC in a biomimetic device occurs at a late stage of colonisation
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Antibiotic Susceptibility of Salmonella using EbbaBiolight supplemented agar
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Uncovering bacteria & funghi interactions
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Tracking of biofilm formation in Burkholderia
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Tracking fungal biofilm formation
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Watching bacterial cities grow
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Are antimicrobial peptides ending the Antibiotic Crisis?
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EbbaBiolight-like molecule for detection and quantification of bacteria
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EbbaBiolight-like molecule detects biomarker for recurrent infection
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EbbaBiolight-like molecules reveals Salmonella biofilm secrets
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EbbaBiolight NBIC Interview
Optotracing with EbbaBiolight
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Antimicrobial peptides for targeting bacterial resistance
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EbbaBiolight fluorescence spectra
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Testimonial - Andrea Sass
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Testimonial - Cameron Croft
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Testimonial - Herve Straub
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What is the role of biofilms in urinary tract infections?
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Opto-electronically active Materials for Infection Detection and Control
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Chemical sensors enable scientists to watch bacterial cities grow
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Discovering an Antibiofilm Therapy for Urinary Tract Infections
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Optotracers - multifunctional fluorescent tracers
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Optotracing for detection & quantification of Staphylococci
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Agneta Richter-Dahlfors explains why biofilm is so important
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Cellulose in urine
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EbbaBiolight-like Molecule used for detection of cellulose in urine
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We named our EbbaBiolight molecules after their peak emission wavelength when they are bound to their target. That means, when EbbaBiolight is bound to a target, it will emit fluorescence at peak emission indicated by the number associated with its name.
To view the excitation and emission spectra, please select your EbbaBiolight below :
2024
- Ray, Sanhita, Susanne Löffler, and Agneta Richter-Dahlfors. 2024. “High-Resolution Large-Area Image Analysis Deciphers the Distribution of Salmonella Cells and ECM Components in Biofilms Formed on Charged PEDOT:PSS Surfaces.” Advanced Science. https://doi.org/10.1002/advs.202307322.
2023
- Antypas, Haris, Tianqi Zhang, Ferdinand X Choong, Keira Melican, and Agneta Richter-Dahlfors. 2023. “Dynamic Single Cell Analysis in a Proximal-Tubule-on-Chip Reveals Heterogeneous Epithelial Colonization Strategies of Uropathogenic Escherichia Coli Under Shear Stress.” FEMS Microbes 4: 1–12. https://doi.org/10.1093/femsmc/xtad007.
- Richter-Dahlfors, Agneta, Elina Kärkkäinen, and Ferdinand X. Choong. 2023. “Fluorescent Optotracers for Bacterial and Biofilm Detection and Diagnostics.” Science and Technology of Advanced Materials 24: 2246867. https://doi.org/10.1080/14686996.2023.2246867.
- Coppens, Bart, Tom E R Belpaire, Ji Rí Pe, Hans P Steenackers, Herman Ramon, and Bart Smeets. 2023. “Anomalous Diffusion of Nanoparticles in the Spatially Heterogeneous Biofilm Environment.” ISCIENCE 26: 106861. https://doi.org/10.1016/j.isci.2023.106861.
- Lorenz, Kairi, Liis Preem, Kadi Sagor, Marta Putrinš, Tanel Tenson, and Karin Kogermann. 2023. “Development of in Vitro and Ex Vivo Biofilm Models for the Assessment of Antibacterial Fibrous Electrospun Wound Dressings.” Molecular Pharmaceutics 20 (February): 1230–46. https://doi.org/10.1021/acs.molpharmaceut.2c00902.
- Shaikh, Nijamuddin, and Karishma S Kaushik. 2023. “Title Optotracers to Study Biofilms in Host-Relevant Systems and Non-Conventional Treatments Using Staphylococcus Aureus Wound Biofilms as a Case Study.” BioRxiv. https://doi.org/10.1101/2023.02.02.526914.
2022
- Koch, Maximilian, Victor Palarie, Lisa Koch, Andreas Burkovski, Manuel Zulla, Stefan Rosiwal, and Matthias Karl. 2022. “Preclinical Testing of Boron-Doped Diamond Electrodes for Root Canal Disinfection—a Series of Preliminary Studies.” Microorganisms 10 (April). https://doi.org/10.3390/microorganisms10040782.
- Kärkkäinen, Elina, Saga G. Jakobsson, Ulrica Edlund, Agneta Richter-Dahlfors, and Ferdinand X. Choong. 2022. “Optotracing for Live Selective Fluorescence-Based Detection of Candida Albicans Biofilms.” Frontiers in Cellular and Infection Microbiology 12 (September). https://doi.org/10.338
- Sass, Andrea, Ian Vandenbussche, Barbara Bellich, Paola Cescutti, and Tom Coenye. 2022. “Pellicle Biofilm Formation in Burkholderia Cenocepacia J2315 Is Epigenetically Regulated Through WspH, a Hybrid Two-Component System Kinase-Response Regulator.” Journal of Bacteriology 204. https://doi.org/10.1128/jb.00017-22.
- Pham, Le Hoang Phu, Mariliz Colon-Ascanio, Jin Ou, Khanh Ly, Piao Hu, John S. Choy, and Xiaolong Luo. 2022. “Probing Mutual Interactions Between Pseudomonas Aeruginosa and Candida Albicans in a Biofabricated Membrane-Based Microfluidic Platform.” Lab on a Chip, October, 4349–58. https://doi.org/10.1039/d2lc00728b.
- Eckert, Johannes A., Ming Rosenberg, Mikael Rhen, Ferdinand X. Choong,and Agneta Richter-Dahlfors. 2022. “An Optotracer-Based Antibiotic Susceptibility Test Specifically Targeting the Biofilm Lifestyle of Salmonella.” Biofilm 4 (December). https://doi.org/10.1016/j.bioflm.2022.100083.
2021
- Rodríguez-Rojas, Alexandro, Desiree Y. Baeder, Paul Johnston, Roland R. Regoes, and Jens Rolff. 2021. “Bacteria Primed by Antimicrobial Peptides Develop Tolerance and Persist.” PLoS Pathogens 17: 1–30. https://doi.org/10.1371/JOURNAL.PPAT.1009443.
- Merkl, Padryk, Marie Stephanie Aschtgen, Birgitta Henriques-Normark, and Georgios A. Sotiriou. 2021. “Biofilm Interfacial Acidity Evaluation by pH-Responsive Luminescent Nanoparticle Films.” Biosensors and Bioelectronics 171: 112732. https://doi.org/10.1016/j.bios.2020.112732.
- Choong, Ferdinand X., Smilla Huzell, Ming Rosenberg, Johannes A. Eckert, Madhu Nagaraj, Tianqi Zhang, Keira Melican, Daniel E. Otzen, and Agneta Richter-Dahlfors. 2021. “A Semi High-Throughput Method for Real-Time Monitoring of Curli Producing Salmonella Biofilms on Air-Solid Interfaces.” Biofilm 3: 100060. https://doi.org/10.1016/j.bioflm.2021.100060.
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