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Carbotrace

Carbotrace Mix&Try – Test Kit for Getting Started

Optotracers are structure-responsive fluorescent molecules which produce unique spectral signatures that can be used to identify specific target molecules. The Carbotrace Mix&Try kit contains five optotracer molecules to test and select which is best suited for your research and experiments. To image our optotracers, you may use any type of fluorescence microscopy equipment (confocal or epifluorescence) and to analyse the spectral signature, you may use a spectrophotometer with fluorescence detection capability or a microscope capable of spectral imaging.

Following optotracer molecules are included in the Carbotrace Mix&Try Kit:

  • Carbotrace 480 (blue) is excited between 405-458 nm and emission is detected between 470–550 nm.
  • Carbotrace 520 (green) is excited between 405-488 nm and emission is detected between 500-600 nm.
  • Carbotrace 540 (yellow) is excited between 430-500 nm and emission is detected between 530-600 nm.
  • Carbotrace 630 (orange) is excited between 458-514 nm and emission is detected between 600-650 nm.
  • Carbotrace 680 (red) is excited between 530-565 nm emission is detected between 600-800 nm.

Carbotrace Mix&Try is available containing 10 µL of each optotracer molecule. Please contact us for custom options.

contact us for custom options.

Carbotrace 680 – red optotracer molecule for spectral imaging of carbohydrates

Optotracing enables direct identification of carbohydrates.

Examples of use:

  • measurement of cellulose content in a sample
  • anatomical mapping of cellulose in plant tissues

To excite the fluorescence of Carbotrace 680, and thereby identify carbohydrate structures in a sample, use any type of fluorescence microscopy equipment (confocal or epifluorescence) or a spectrophotometer with fluorescence detection capability.

Carbotrace 680 has an optical spectrum that allows custom settings within the detection range of 600-800 nm and an excitation range of 530-565 nm. Emission can be detected at 680 nm using the standard PI (Propidium Iodide), mCherry or Cy3.5 filter set and exci- tation is achieved using the 561 nm laser line.

Carbotrace 680 is available in 50, 100, 150 and 200 µL with a volume discount and is shipped in vials containing 50 µL each. Please contact us for custom options.

contact us for custom options.

Carbotrace 630 – orange optotracer molecule for spectral imaging of carbohydrates

Optotracing enables direct identification of carbohydrates.

Examples of use:

  • measurement of cellulose content in a sample
  • anatomical mapping of cellulose in plant tissues

To excite the fluorescence of Carbotrace 630, and thereby identify structures in sample, use any type of fluorescence microscopy equipment (confocal or epifluorescence) or a spectrophotometer with fluorescence detection capability.

Emission for Carbotrace 630 can be detected at 630 nm using the standard PI (Propidium Iodide), Cy3, TxRed, mCherry or Cy3.5 filter set and excitation is achieved using the 488 or 514 nm laser line. The optical spectrum allows custom settings within the detection range of 600-650 nm and an excitation range of 458-514 nm.

Carbotrace 630 is available in 50, 100, 150 and 200 µL with a volume discount and is shipped in vials containing 50 µL each. Please contact us for custom options.

contact us for custom options.

Carbotrace 540 – yellow optotracer molecule for spectral imaging of carbohydrates

Optotracing enables direct identification of carbohydrates.

Examples of use:

  • measurement of cellulose content in a sample
  • anatomical mapping of cellulose in plant tissues

To excite the fluorescence of Carbotrace 540, and thereby identify structures in sample, use any type of fluorescence microscopy equipment (confocal or epifluorescence) or a spectrophotometer with fluorescence detection capability.

Emission for Carbotrace 540 can be detected at 540 nm using the standard FITC, GFP or YFP filter set and excited using the 440 nm laser line. The optical spectrum allows custom settings within the detection range of 530-600 nm and excitation range of 430-500 nm.

Carbotrace 540 is available in 50, 100, 150 and 200 µL with a volume discount and is shipped in vials containing 50 µL each. Please contact us for custom options.

contact us for custom options.

Carbotrace 520 – green optotracer molecule for spectral imaging of carbohydrates

Optotracing enables direct identification of carbohydrates.

Examples of use:

  • measurement of cellulose content in a sample
  • anatomical mapping of cellulose in plant tissues

To excite the fluorescence of Carbotrace 520, and thereby identify structures in sample, use any type of fluorescence microscopy equipment (confocal or epifluorescence) or a spectrophotometer with fluorescence detection capability.

Emission for Carbotrace 520 can be detected at 520 nm using the standard FITC or GFP filter set and excited using the 458 or 488 nm laser line. The optical spectrum also allows custom settings within the emission detection range of 500-600 nm and excitation range of 405-488 nm.

Carbotrace 520 is available in 50, 100, 150 and 200 µL with a volume discount and is shipped in vials containing 50 µL each. Please contact us for custom options.

contact us for custom options.

Carbotrace 480 – blue optotracer molecule for spectral imaging of carbohydrates

Optotracing enables direct identification of carbohydrates.

Examples of use:

  • measurement of cellulose content in a sample
  • anatomical mapping of cellulose in plant tissues

To excite the fluorescence of Carbotrace 480, and thereby identify structures in sample, use any type of florescence microscopy equipment (confocal or epifluorescence) or a spectrophotometer with fluorescence detection capability.

Emission for Carbotrace 480 can be detected at 480 nm using the standard DAPI filter set and excited using the 405 nm laser line. The optical spectrum also allows custom settings within the detection range of 470-550 nm and excitation range of 405-458 nm.

Carbotrace 480 is available in 50, 100, 150 and 200 µL with a volume discount and is shipped in vials containing 50 µL each. Please contact us for custom options.

contact us for custom options.

General Information

Carbotrace are optotracer molecules providing means for anatomical mapping of carbohydrate structures in plants and for non-destructive composition analysis of polysaccharides in bio-based materials and biofuels.

The optotracing technology has been developed by a team of researchers at Karolinska Institutet (KI), Royal Institute of Technology (KTH) and Linköping University in Sweden and is a result of many years of cutting-edge nanoscience and organic chemistry research. Optotracing has been defined by the researchers as the use of structure-responsive fluorescent molecules which become highly fluorescent when binding and exhibit unique spectral signatures dependent on the structure of their binding partner. The unique combination of fluorescence and the target-specific signal can be used to clearly identify biomolecules in various types of materials.

Carbotrace optotracers are designed for anatomical mapping of carbohydrate structures in plants and for non-destructive composition analysis of polysaccharides in bio-based materials and biofuels. The structure-responsive optotracer Carbotrace differentiates between different types of glycosidic linkages in carbohydrates.

Spectral Imaging

Identification of Cellulose and Starch Structures in Potato

  • prepare freshly sliced potato (1 mm)
  • dilute Carbotrace 680 1:1000 in PBS (pH 7.4)
  • immerse potatoes in diluted Carbotrace 680 for ca. 30 min
  • wash with PBS
  • mount on microscope slide
  • image with the following excitation/emission settings: Ex: 405 nm Em: 430–450 nm, Ex: 473 nm Em: 490–540 nm, Ex: 535 nm Em: 575–620 nm and Ex: 635 nm Em: 655–755 nm or acquire spectra with a plate reader or spectral microscope.

Carbotrace 680 is a structure-responsive optotracer. That means, applied in a potato sample, it shows a unique spectral signature when binding to cellulose or starch. In cellulose, glucose units are joined by β-1,4-glycosidic bonds and in starch, which consists of amylose & amylopectin, glucose units are joined by α-1,4- and α-1,6-glycosidic bonds. Carbotrace 680 can distinguish between the different glycosidic linkages in these structures.

Quick Facts

Carbotrace optotracers are:

  • Fast
  • Simple and easy to handle
  • Photo- and thermostable
  • Non-toxic
  • Accurate

Applications

Storage

  • Store Carbotrace at 4°C.
  • Use the opened vial within 12 month.

Note

  • Carbotrace is for research use only.
  • Carbotrace is not for diagnostic use or use in humans.
  • Carbotrace is not for resale.

Download Info

Protocol I: Anatomical mapping of cellulose in plant tissues using Carbotrace 680

This protocol describes a non-destructive method of anatomical mapping of cellulose structures in plant tissues.

Solutions and Reagents:

Carbotrace 680 is provided as concentrated solution. The following common reagents are required (not supplied):

  • Phosphate buffered saline (PBS), pH 7.4

Assay Procedure:

  • Prepare thin sections of plant tissue.
  • Dilute Carbotrace 680 in PBS 1:1000.
  • Apply diluted Carbotrace™ generously on the plant tissue. Use enough liquid (ca 0.5 ml) to prevent the sections from drying out during incubation. Incubate for 30 min.
  • Wash 2 x 5 min in PBS.
  • Mount sections for microscopic analysis.

Fluorescence Microscopy:

  • Carbotrace 680: Excite at 535 nm and detect emission using a 575-620 nm bandpass filter. Excitation at 635 nm and detection using a 655-755 nm bandpass filter is also possible. Alternatively, you can use the standard settings for AlexaFluor™635. The broad optical spectrum allows custom settings to be applied, using an excitation range of 530-565 nm and a detection range of 600-800 nm.

Download Protocol

Protocol II: Measurement of cellulose content in a sample using Carbotrace 680

This protocol describes how to use Carbotrace 680 to determine cellulose content in a sample.

Solutions and Reagents:

Carbotrace 680 is provided as concentrated solution. The following common reagents are required (not supplied):

  • Phosphate buffered saline (PBS), pH 7.4
  • 96-well plate (round bottom)
  • Spectrophotometer

Assay Procedure:

  • Prepare a dilution series of your cellulose containing sample im PBS (e.g.: 1, 1:2, 1:5, 1:10, 1:100, 0)
  • Dilute Carbotrace 680 in PBS 1:500.
  • Add 50 µl of each cellulose containing dilution into a well of a 96-well plate.
  • Add 50 µl diluted Carbotrace 680 to each cellulose containing dilution and the blank control.
  • Measure emission using spectrophotometer settings (see 'Spectrophotometer Settings' below).

Spectrophotometer Settings:

  • Carbotrace 680: Excite at 540 nm and collect emission at 680 nm. Optional: Record an emission spectrum (560 - 800 nm) with 540 nm excitation.

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