Cart 0

Carbotrace™

General Information

Carbotrace™ are fluorescent tracer molecules providing means for non-destructive quality assessment of polysaccharides. Carbotrace™ molecules are fluorescent only when bound to their target yielding high signal-to-noise ratio for fluorescence microscopic and spectrophotometric analysis.

Carbotrace™680 is our red fluorescent tracer molecule for cellulose detection.

Carbotrace™680 fluorescence is readily visualized using standard microscopy equipment. Excite at 535 nm and detect emission using a 575-620 nm bandpass filter. Or excite at 635 nm and detect emission using a 655-755 nm bandpass filter. Alternatively, you can use the standard settings for AlexaFluor™635. The optical spectrum of Carbotrace™680 allows custom settings to be applied, using an excitation range of 530-565 nm and a detection range of 600-800 nm.

Spectrophotometric cellulose detection

Carbotrace™ is fluorescent only when bound to its target. Therefore, Carbotrace™ can easily be used for spectrophotometric analysis of cellulose content in various samples. We used a dilution series of nanocellulose and measured the emission at 680 nm under excitation with 560 nm to detect nanocellulose in a pure sample. Relative amounts of cellulose can be determined using this method.

Anatomical mapping of cellulose

Carbotrace™ can be used for non-destructive analysis of cellulose distribution in various samples. Anatomical mapping of cellulose in plant tissue slices was performed using confocal microscopy. Excitation was performed at 635 nm and emission was collected using a 655-755 nm bandpass filter. Large multi-faced cell walls of medullary parenchyma cells can be observed but no intracellular organelles - consistent with the specificity for cellulose. Using this technique, intricate details on the inner face of cell walls are visualized.

Carbotrace™680 is available as ex vivo variant in 50 µl and 100 µl aliquots. Please contact us for customs options.
contact us for custom options.

Carbotrace™

  • are provided as ex vivo variant in volumes of 50 µl and 100 µl
  • are diluted 1000-fold
  • are non-toxic
  • are photo- and thermostable

Applications

Storage

  • Store Carbotrace™ at 4°C
  • Use the opened vial within 12 month

Note

  • Carbotrace™ is for research use only.
  • Carbotrace™ is not for use in animals or humans.
  • Carbotrace™ is not for resale.
  • Carbotrace™ is a trademark belonging to Ebba Biotech AB

Download Info

Protocol I: Anatomical mapping of cellulose in plant tissues

This protocol describes a non-destructive method of anatomical mapping of cellulose structures in plant tissues.

Solutions and Reagents:

Carbotrace™ is provided as concentrated solution. The following common reagents are required (not supplied):

  • Phosphate buffered saline (PBS), pH 7.4

Assay Procedure:

  • Prepare thin sections of plant tissue.
  • Dilute Carbotrace in PBS 1:1000.
  • Apply diluted Carbotrace™ generously on the plant tissue. Use enough liquid (ca 0.5 ml) to prevent the sections from drying out during incubation. Incubate for 30 min.
  • Wash 2 x 5 min in PBS.
  • Mount sections for microscopic analysis.

Fluorescence Microscopy:

  • Carbotrace™680: Excite at 535 nm and detect emission using a 575-620 nm bandpass filter. Excitation at 635 nm and detection using a 655-755 nm bandpass filter is also possible. Alternatively, you can use the standard settings for AlexaFluor™635. The broad optical spectrum allows custom settings to be applied, using an excitation range of 530-565 nm and a detection range of 600-800 nm.

Download Protocol

Protocol II: Measurement of cellulose content in a sample

This protocol describes how to use Carbotrace™ for tracking cellulose content in a sample.

Solutions and Reagents:

Carbotrace™ is provided as concentrated solution. The following common reagents are required (not supplied):

  • Phosphate buffered saline (PBS), pH 7.4
  • 96-well plate (round bottom)
  • Spectrophotometer

Assay Procedure:

  • Prepare a dilution series of your cellulose containing sample im PBS (e.g.: 1, 1:2, 1:5, 1:10, 1:100, 0)
  • Dilute Carbotrace™ in PBS 1:500.
  • Add 50 µl of each cellulose containing dilution into a well of a 96-well plate.
  • Add 50 µl diluted Carbotrace™ to each cellulose containing dilution and the blank control.
  • Measure emission using spectrophotometer settings (see 'Spectrophotometer Settings' below).

Spectrophotometer Settings:

  • Carbotrace™680: Excite at 540 nm and collect emission at 680 nm. Optional: Record an emission spectrum (560 - 800 nm) with 540 nm excitation.

Download Protocol

Download
Download
Download
Download
Terms & Conditions

For detailed information on Terms & Conditions, please click the link below.

Terms & Conditions

Privacy Policy

To read more how we handle personal information, please click the link below.

Privacy Policy & Cookie Policy

Support

If you have any questions related to our products or services, please contact us.

info@ebbabiotech.com