Amytracker
Amytracker are small fluorescent molecules for detection of protein aggregates.
Five Amytracker variants are available. All Amytracker variants are designed to bind to the Congo red binding pocket on the amyloid fibril. A minimum of eight in-register parallel-β-strands are required for binding. The Amytracker variants differ in affinity and spectral properties. As Amytracker are structural markers, you can achieve reliable fluorescent labeling of amyloids derived from a variety of amyloidogenic proteins or peptides from different species.
Amytracker are suitable for detecting amyloids in fresh or fixed tissue sections and cells. It is possible to use them for fibrillation assays and for systemic injection in vivo. They are exceptionally photo- and thermostable and allow for easy handling in any application. Amytracker work in a wide range of salt and pH conditions. When the pH is altered during the experiment, pH controls should be included. Amytracker can be used with fluorescence plate readers, fluorescence microscopes and confocal laser scanning microscopes, fluorescence life time imaging, fluorescence cytometry, Total internal reflection fluorescence (TIRF) microscopy and Multiphoton microscopy.
Store your Amytracker product in the fridge and use the opened container within 12 months. Amytracker is for research use only and is not for resale.
Amytracker Mix&Try Kit is our recommended option for starting out with using Amytracker. It contains a sample volume (10 µL) of each variant. Testing each variant will allow you to determine which Amytracker is best suited for your experiments and available instruments.
All Amytracker variants label Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. The optotracers are exceptionally photostable and fluorogenic. The variants differ with regards to affinity, cellular uptake and excitation and emission wavelengths (see Table below). When bound to a target, Amytracker can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Contact us to learn more about Amytracker applications.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
Amytracker 480 | 420 nm | 480 nm | DAPI |
Amytracker 520 | 460 nm | 520 nm | FITC, GFP |
Amytracker 540 | 480 nm | 540 nm | FITC, GFP, YFP |
Amytracker 630 | 520 nm | 630 nm | PI, Cy3, TxRed, mCherry, Cy3.5 |
Amytracker 680 | 530 nm | 680 nm | PI, mCherry, Cy3.5 |
Amytracker 680 is our red optotracer for labeling protein aggregates with repetitive arrangement of β-sheets. It labels Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. Specifically, Amytracker 680 has been used to study amyloid formation during abnormal coagulation, Lewy body formation in a seeding based neuronal model, accumulation of misfolded proteins in the nucleolus and intracerebral formation of Aβ plaques using multiphoton microscopy. Contact us to learn more about Amytracker applications.
As all our optotracers, Amytracker 680 is exceptionally photostable and fluorogenic. When bound to a target, Amytracker 680 can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
Amytracker 680 | 530 nm | 680 nm | PI, mCherry, Cy3.5 |
Amytracker 680 is available in four different formulations (See volumes and prices in the drop-down list below):
- Aqueous: 1 mg/ml solution in ultrapure water. The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
- DMSO: 1 mg/ml solution in DMSO to prevent solvent evaporation. The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations.
- Solid: 1 mg solid lyophilised in a sterile injection bottle. We recommend dilution to 4 mg/ml in physiological saline followed by intravenous injection with a total dose of 5 mg/KG.
- Drop&Shine: 5 ml ready-to-use product in mounting medium. Ideal for use in tissue sections. Add a some Drop&Shine and mount your slide to detect amyloids within minutes.
Amytracker 630 is our orange fluorescent optotracer for labeling protein aggregates with repetitive arrangement of β-sheets. It labels Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. Contact us to learn more about Amytracker applications.
As all our optotracers, Amytracker 630 is exceptionally photostable and fluorogenic. When bound to a target, Amytracker 630 can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
Amytracker 630 | 520 nm | 630 nm | PI, Cy3, TxRed, mCherry, Cy3.5 |
Amytracker 630 is available as 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (See volumes and prices in the drop-down list below):
Amytracker 540 is our yellow fluorescent optotracer molecule for labeling protein aggregates with repetitive arrangement of β-sheets. It labels Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. Contact us to learn more about Amytracker applications.
As all our optotracers, Amytracker 540 is exceptionally photostable and fluorogenic. When bound to a target, Amytracker 540 can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
Amytracker 540 | 480 nm | 540 nm | FITC, GFP, YFP |
Amytracker 540 is available as 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (See volumes and prices in the drop-down list below):
Amytracker 480 is our blue fluorescent optotracer for labeling protein aggregates with repetitive arrangement of β-sheets. It labels Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. Specifically, Amytracker 480 has been used to study amyloid formation during abnormal coagulation. Contact us to learn more about Amytracker applications.
As all our optotracers, Amytracker 480 is exceptionally photostable and fluorogenic. When bound to a target, Amytracker 480 can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table.
Exmax | Emmax | Recommended filter-sets | |
---|---|---|---|
Amytracker 480 | 420 nm | 480 nm | DAPI |
Amytracker 480 is available as 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (See volumes and prices in the drop-down list below):
Protocol I: Staining of protein aggregates in tissue sections
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Protocol II: Staining of protein aggregates with Amytracker and antibody co-stain
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Protocol III: Live-cell imaging
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Protocol IV: Fibrillation assay
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Protocol I: Staining of protein aggregates in tissue sections
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Protocol II: Staining of protein aggregates with Amytracker and antibody co-stain
Read more →
Protocol III: Live-cell imaging
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Protocol IV: Fibrillation assay
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Amyloidogenicity of SARS-CoV-2 spike protein
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Lewy body formation in seeding based neuronal models
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Prolonged stress leads to accumulation of misfolded proteins in the nucleolus
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Amytracker can be used for intracerebral multiphoton microscopy
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The clue to detect multiple systems atrophy?
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Anomalous fibrin amyloid formation
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Amyloids in type-2 diabetes
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Advanced imaging
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Protein engineering for better PET radioligands
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Artifical amyloids
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Amytracker and Long Covid
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Amytracker and the age-pigment Lipofuscin
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Amytracker for the study of tau aggregates
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Amytracker for studying α-synuclein aggregates
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When proteins get out of shape
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Are amyloid structures in abnormal blood clots a risk factor for amyloidosis?
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Phase separation and protein aggregation
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Amytracker fluorescence spectra
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Amytracker compared to Congo Red
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Does Amytracker bind unspecifically?
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Amytracker for use in various tissues and species
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Fixation technique for Amytracker
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Amytracker for detection of Amyloidosis
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Amytracker binding
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Amytracker compared to Thioflavin
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How should I dilute Amytracker?
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Filter sets to detect Amytracker fluorescence
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The staining didn’t work, what should I do?
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Amyloids - the dark matter of biology
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Amytracker for amyloid staining
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Amytracker to investigate amyloid formation
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Amytracker for superresolution microscopy
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Amytracker for live cell Imaging
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In vivo amyloid staining and intravital imaging
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Testimonial about Amytracker 680 - Adam Kreutzer
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Testimonial about Amytracker 540 - Keiza Jack
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Testimonial about Amytracker 680 - Jaakko Sarparanta
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Testimonial about Amytracker 520 - Megg Garcia
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Amyloid fibril polymorphism in proteinopathies
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Consequences of coagulation in health and disease: The use of fluorescent markers
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Optotracers - multifunctional fluorescent tracers
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Fluorescence microscopy techniques using Amytracker-like molecules
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Peter Nilsson develops multifunctional tools for diagnosis and therapy
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We named our Amytracker molecules after their peak emission wavelength when they are bound to their target. That means, when Amytracker is bound to a target, it will emit fluorescence at peak emission indicated by the number associated with its name.
To view the excitation and emission spectra, please select your Amytracker below :
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