Dr. Andrea Sass about EbbaBiolight 680:
"In the Laboratory of Pharmaceutical Microbiology, Ghent University, we used EbbaBiolight 680 for visualizing extracellular matrix in pellicles formed by Pseudomonas aeruginosa. We found that the product labels pellicle matrix of P. aeruginosa specifically, bacterial cells were not labelled. The method revealed structural differences between pellicles of different strains and mutants, and gave us valuable insights into the pellicle structure. We were particularly impressed by how easy the optotracer solution is to use, just dilute it in phosphate buffered saline, or in medium. The cells can be grown in the presence of the optotracer, which means they can be microscopically observed without an additional staining step which could disrupt the structures. If necessary, post-labelling of pellicles was performed and also worked very well for us. There was no need for incubation time, the pellicles were labelled immediately. The fluorescence emitted by the optotracers is very stable, we did not observe any photobleaching during fluorescence microscopy. Overall, EbbaBiolight is a very useful additional tool for investigating the extracellular matrix of P. aeruginosa."
Dr. Andrea Sass, Laboratory of Pharmaceutical Microbiology, Ghent University, Belgium
Labeling of Pseudomonas aeruginosa PAO1 and PAO14 biofilm with EbbaBioligth 680. The overlay with brightfield images show that the pellicle biofilm is labeled with EbbaBiolight 680 (red) but not single bacteria. Images were kindly provided from Dr. Andrea Sass and Prof. Tom Coenye, Laboratory of Pharmaceutical Microbiology, Ghent University
Dr. Ferdinand X. Choong about EbbaBiolight 680:
"EbbaBiolight 680 has been a great help to our research at AIMES. The ease of use and ability to analyze bacterial cultures and biofilms as they grow has allowed us to gain valuable insights previously not possible. Today, we continue to use this fluorescent tracer in our work to develop deeper, better understandings of infections in healthcare. "
Dr. Ferdinand X. Choong is Assistant Professorat Karolinska Institutet and Team Leader at the Center for the Advancement of Integrated Medical and Engineering Sciences (AIMES) in Stockholm, Sweden. AIMES is a new center promoting interdisciplinary research, innovation, implementation and entrepreneurship and is funded by KTH Royal Institute of Technology and Karolinska Institutet.
Cameron Croft about EbbaBiolight 680:
"Our group has used crystal violet in the past to quantify the total biomass of E. coli biofilms, with mixed results. In addition to an end-point assay, we were looking for a method to quantify the development of biofilm throughout growth. In our hands, EbbaBiolight 680 has proven to be reliable for monitoring indications of early biofilm development in E. coli.”
Cameron Croft, Postgraduate student in Dr. David Summers group at Department of Genetics, University of Cambridge, Cambridge, UK.
Labeling of E. coli biofilm with EbbaBiolight 680. The overlay with brightfield images shows that biofilm is labeled with EbbaBiolight 680 (red) but not single bacteria. Images were kindly provided from Cameron Croft, Postgraduate student in Dr. David Summers group at Department of Genetics, University of Cambridge, Cambridge, UK.
Hervé Straub about EbbaBiolight 680:
Hervé Straub is a PhD student at Empa - Swiss Federal Laboratories for Materials Science and and Technology, St. Gallen, Switzerland and is working on the establishment of an automatized microfluidic platform to study bacterial biofilm formation in real-time by optical microscopy.
Pseudomonas aeruginosa (PAO1) grown in a microfluidic chamber with M9 medium for several days, labeled with EbbaBiolight 680. Red fluorescence channel was overlayed with bright field at 40X magnification. Image kindly provided from Hervé Straub, PhD student at Empa - Swiss Federal Laboratories for Materials Science and and Technology, St. Gallen, Switzerland