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Prof. Ulrica Edlund about Carbotrace 540:

"We were studying the biochemical composition of Ulva lactuca (a.k.a. Ulva fenestrata), which is still not fully known. Our hypothesis, and we wanted to prove, was the presence of cellulose and we knew that optotracing was probably the best method for analysis to distinguish cellulose from other glucans, but not which optotracing molecule would work the best. So we simply took a few of the optotracers we had already used plus the newest available variant [Carbotrace 540 from Ebba Biotech] and that one proved to work best of all! We saw a clear redshift in the excitation spectra and a specific peak, confirming the presence of cellulose nanofibrils. So we have proven the presence of cellulose in Ulva and that the xylose detected in other analyses must stem from another saccharide co-existing with cellulose. Our studies will now continue to try to more fully understand the structure of Xyloglucan."

Ulrica Edlund is Professor in Polymer technology at KTH Royal Institue of Technology and Vice Director at the Center for the Advancement of Integrated Medical and Engineering Sciences (AIMES) at Karolinska Institutet in Stockholm, Sweden.

Testimonial given on August 13th, 2021


Dr. Ferdinand X. Choong about Carbotrace 680:

"It really made carbohydrate detection so much easier! Thinking back on how to detect starch you had to use iodine solutions - that was kind of messy - and now Carbotrace 680 just works - simple and accurate."

Dr. Ferdinand X. Choong is Assistant Professorat Karolinska Institutet and Team Leader at the Center for the Advancement of Integrated Medical and Engineering Sciences (AIMES) in Stockholm, Sweden. AIMES is a new center promoting interdisciplinary research, innovation, implementation and entrepreneurship and is funded by KTH Royal Institute of Technology and Karolinska Institutet.

Testimonial given on May 21st, 2021


Prof. Ulrica Edlund about Optotracing:

Ulrica Edlund is Professor in Polymer technology at KTH Royal Institue of Technology and Vice Director at the Center for the Advancement of Integrated Medical and Engineering Sciences (AIMES) at Karolinska Institutet in Stockholm, Sweden.

Optotracing in biorefinement of Canola Straw. Thin sections of canola straw were prepared for non-destructive optotracing analysis by fluorescence microscopy. Optotracing shows cellulose yellow and lignin blue-turquoise. The images were kindly provided by Prof. Ulrica Edlund.

Testimonial given on April 29th, 2021


Dr. Liudmila Kozlova about Carbotrace 680:

"We have used Carbotrace 680 to resolve the cellular structure of studied plants. It is possible due to the high content of cellulose in plant cell walls. Moreover, the fluorescence intensity of Carbotrace 680 can be used for the determination of cell wall thickness and, hence, as the normalization factor for quantification of labelling/staining by other cell wall-specific agents. Carbotrace 680 may be a better choice for cell wall thickness quantification than Calcofluor White in the case you are studying plant species that display high autofluorescence at UV irradiation (i.e. almost all Monocots). Carbotrace 680 also may help if for some reasons you cannot use UV excitation and/or blue light detection. Our experience with that molecule was just great. We definitely will use it again."

Dr. Liudmila Kozlova, Kazan Scientific Center of the Russian Academy of Sciences, Kazan Institute of Biochemistry and Biophysics, Kazan, Russia

Fluorescence micrographs of maize root cross-sections stained with Carbotrace 680. The images were kindly provided by Dr. Anna Petrova, Kazan Scientific Center of the Russian Academy of Sciences, Kazan Institute of Biochemistry and Biophysics, Kazan, Russia.

Testimonial given on March 25th, 2021


Tharagan Kumar about Carbotrace 680:

Tharagan Kumar is doctoral student at KTH Royal Institute of Technology, Division of Nanobiotechnology located at SciLifeLab in Solna, Sweden. Tharagan sent us an image about how he was using Carbotrace 680 in his work.

Optimization of an immobilization matrix made of cellulose nanofibrils in a microfluidic device. The images were kindly provided by Tharagan Kumar, KTH Royal Institue of Technology, Division of Nanobiotechnology.

Testimonial given on April 29th, 2021