β-2-microglobulin is a protein present in all nucleated cells and is elevated in many tumors or inflammatory diseases. It is well known that the protein has a propensity for aggregation. A study published in the journal Immunity by Hofbauer et al. has investigated the role of β-2-microglobulin aggregates on the activation of inflammatory processes in Multiple Myeloma. Amtracker 480 has been used in the study to detect amyloid aggregates of β-2-microglobulin in live macrophages and detected by Flow Cytometry.

Do you want to learn more? Read the publication here.

Image: The structure of β-2-microglobulin in its native and fibrillar states. Image from Figure 4 AB by Iadanza et al. (2018) Nature Communications, 9(1), 4517 (CC BY 4.0).

Did you just purchase your Amytracker product and wondering about the perfect dilution? No worries! At Ebba Biotech, our dedicated team is here to help you every step of the way. If you have any questions or need further assistance, please don’t hesitate to reach out to us here. We’ve got you covered!

A publication in iScience by Jitka Petrlova from Artur Schmidtchen’s group at Lunds University discovered an interesting connection between Lipopolysaccharides (LPS) and the wound healing process. The researchers found that LPS, the outer membrane component of gram-negative bacteria, can induce protein aggregation in wounds and isolated wound fluids. This process would, in turn, reduce inflammation and contribute to wound healing. To detect the formation of amyloid-like aggregates, they used Amytracker 680 and observed under a fluorescent microscope.

Do you want to learn more details about how Amytracker was used in this publication? Click here

Image: Protein aggregation in acute wound fluids (AWFs) in the presence and absence of LPS. Fluorescence microscopy images of AWFs +/- LPS (100 μg/ml) labeled with Amytracker 680 in red. An increase in the Amytracker 680 signal indicates aggregates in AWFs after the addition of LPS. Image from Figure 2D by Petrlova et al. (CC BY 4.0)

Did you ever wonder what our users think about our products?

Or perhaps you’re looking to learn more before making a purchase decision? Look no further! Check out the testimonials from our customers to gain valuable insights into their experiences.

Read the full testimonial from Prof. Chiti and Others.

Happy Kardemummabullens dag (Cardamom Bun Day for our non-swedish speakers)

Ebba Biotech - just like all Swedes - takes sweet treats seriously and as such finds it pertinent to celebrate the sweet small things in life. As such, our team came together to enjoy some classic Swedish fika in the sun.

At Ebba Biotech, our slogan "By Scientists for Scientists" isn’t just a tagline, it’s genuinely our truth. We’re not just selling our optotracer products; we intimately understand the ins-and-outs of the technology. This profound knowledge ensures our solutions are tailored to meet the needs of our customers and fellow scientists.

Don’t hesitate to contact our team with any inquiries you might have. We’re here to help! Contact us here

We are excited to be joined by Dr. Frederik Zitzmann from the University of York presenting his research using Carbotrace. The webinar highlights how adopting novel methods - such as visualization and analytical techniques with Carbotrace - is revolutionizing the biorefinery industry working towards a more sustainable future with more sustainable material.

What’s the secret to business success?

In our case, strong customer relations is the key. Engaging with our customers isn’t just a strategy, it’s our passion. Your feedback fuels our growth and helps us assist you better. Please don’t hesitate to reach out to our team!

Contact us here

👏 That's a wrap on the Microbiology Society Annual Conference 👏

Thank you to all the participants for making this such a successful conference for Emile and Smilla! It was fantastic to introduce you all to the EbbaBiolight molecules and optotracing technology 🦠🔬 To all our current customers, thank you for showcasing your work all throughout the conference - special shout out to longtime user Ashraf Zarkan presenting Urinary Extracellular Vesicles against E.coli biofilms using EbbaBiolight 🧫 We were also so pleased to see that you all enjoyed all our merch (👀👜 tote bags 👜👀) so that you too can show off Ebba Biotech wherever you go! For anyone who missed out on the "Passport to Prizes" question, EbbaBiolight has been featured in 1️⃣ 4️⃣ publications since its launch in the summer of 2020 🎉 But let us know if you want to be part of increasing this number moving forward 😜

🌟 Do you ever wonder what users think about EbbaBiolight? 💭 Or perhaps you’re looking to learn more before making a purchase decision? 🛍

Check out our testimonials on our website to gain valuable insights into the experiences of our customers.💡

Did you know that, according to the Parkinson’s Foundation, more than 10 million people worldwide are living with Parkinson’s disease? Here at Ebba Biotech we are constantly in discussions with academics and industries that are actively researching and developing therapies to tackle Parkinson’s disease using our Amytracker product family as a visualisation tool.

One company we would like to highlight is reMYND! The team at reMYND have been utilising the Amytracker 520 variant to enhance their imaging in mice - just look at the images below and hear from reMYND themselves!

“Focusing on the top image, we peer into the injection site (dorsal striatum), where pathogenic fibrils lurk (using DAPI as a nuclear stain). But the story doesn’t end there -as the pathology spreads throughout the mouse brain, it infiltrates crucial regions like the amygdala (lower image). With exceptional precision, Amytracker detects Lewy Bodies and Lewy Neurites, pivotal entities in Parkinson’s disease pathology. Paving the way for quantification of drug efficacy or target engagement studies for our clients.”

We are excited to follow reMYND as they continue to create positive change in the neurodegenerative field. To learn more about their work click here

A repeat expansion in the C9orf72 gene is the most frequent cause of ALS and FTD. Researchers from Max Planck Institute of Biochemistry and University Medical Center Gröningen revealed the molecular mechanism behind Dipeptide Repeat Proteins (DRP) cytotoxicity in their publication in the journal eLife. They used Amytracker 680 to localize and characterize both cytoplasmic and nuclear DRP aggregates.

Image:🔬HEK293 cells transfected with the indicated constructs and labeled with Amytracker 680. (AmyT in red, Poly-GA DPRs in green, nuclear membrane indicated by white dashed lines). Frottin et al. (Fig. 1D) (CC BY 4.0)

Our products are featured in over 65 publications in various high-impact journals, we’re proud to share our contributions to advancing scientific knowledge! From groundbreaking discoveries to innovative technologies worldwide.

Curious to learn more? Reach out to us today!

Yesterday, our COO Smilla connected with lots of engaged students at the Mingle Event for the KI Careers Internship Program, showcasing the exciting internship opportunities at Ebba Biotech. 👨‍🔬

Today, we honor, celebrate, and appreciate the incredible women making a difference around the world.🌎💖

Did you know that Ebba Biotech was founded by a visionary woman - Prof. Agneta Richter-Dahlfors? 🤔 But there’s more! We take immense pride in being a company where exceptional women lead the team 👯‍♀️ and run the office 👭 in order to innovate and push Ebba Biotech forward. 💪🌟

Finally, the news is out! Today marks the launch of our eagerly awaited updated product catalog, featuring three new formulations for all product families and variants: 🌟 DMSO, Drop&Shine, and Solid.🚀

But that’s not all! Remember to explore our original formulation (ultrapure water) under a new name - Aqueous - as well as our top-notch testing service. 👨‍🔬

During the past month, we have enjoyed having Alejandro as our Guest Ph.D. student from the KI Careers Service program.👨‍🔬 He has been able to venture into the world of Life Science start-ups by getting first-hand experience in sales and marketing alongside active product development work in the lab.

It has been a genuine pleasure to get to know you and we look forward to following your career moving forward. 🚀 Thank you, Ale, for your valuable contributions, and we hope our paths cross again in the future. 🌟

Small protein aggregates (below 450 nm) are highly toxic as they are able to penetrate the cell membrane. Their small size makes these aggregates especially hard to study. 💡 With Amytracker, however, you can finally see them! Michael J. Morten and colleagues came up with a new approach that involves using Amytracker 630 and super-resolution microscopy to resolve aggregates involved in neurodegenerative disorders. In their experiments, Amytracker 630 performed much better than conventional staining methods, such as Thioflavin! 🏅

"We show that Amytracker 630 (AT630), a commercial aggregate-activated fluorophore, has outstanding photophysical properties that enable super-resolution imaging of α-synuclein, tau, and amyloid-β aggregates, achieving ∼4 nm precision"

🔬Image: Cell-penetrating aggregate species in HEK cells (Live super-resolution imaging by SMLM.) AmyT 630 (red), Proteasome foci (green). Typical foci-aggregate colocalizations are shown in the zoomed-in images. (white scale bars: 2 μm l and 200 nm), Morten et al. (Fig. 3C) (CC BY 4)

🇸🇪 Today, the entire team was invited to Academic Work to celebrate Shrove Tuesday - or as we call it here in Sweden - Semmeldagen!☕️

Embracing the fika-culture to its fullest by indulging in a cardamom-infused bun filled with a sweet almond paste and whipped cream🍰

Thanks to Malin Kristoffersson for the invitation.🙌

At Ebba Biotech, we believe in more than just providing biotech solutions - we are all about building lasting connections and ensuring customer satisfaction!🌟

🤝 Our team is the heart of Ebba Biotech, driven by a shared commitment to delivering not only fantastic products but also providing exceptional customer support. Every member will go the extra mile to understand and troubleshoot customer issues alongside our users to ensure seamless integration of the optotracer products in labs worldwide.🔬🌈

Reach out and chat with our team members to see how you can get started with optotracers in your lab🤩

What causes the accumulation of toxic amyloid aggregates? An article by Rimal et al. published in the journal Acta Neuropathologica Communications might have found the answer to this question. Their work shows that a defective ribosome quality control (RQC) causes the C-terminal fragment (APP.C99) of the Amyloid precursor protein (APP) to accumulate. This might be one of the earliest pathogenic events of amyloid aggregation. Labeling such aggregates in HeLa cells with Amytracker 680 shows that they have amyloid-like properties.

Image:🔬 Ribosome stall-induced aberrant APP.C99 seeds amyloid ß-42 aggregation in HeLa cells after addition of Aß42 AT tails. Fluorescent microscope images showing aggregation of Aß42 as detected with the aggregation-specific dye Amytracker (in blue). Rimal et al. Fig. S6D (CC-BY-4.0).

🌟 Introducing our newest recruit Alejandro Lastra Romero! 🌟

We are excited to have Alejandro as our Guest PhD student for the entirety of February! 🎉🤝 As a Guest PhD student, Alejandro will be learning about the day-to-day operations of a start-up like Ebba Biotech by sampling all the various roles within our team. 🚀

👨‍🔬 About Alejandro

Alejandro is a biochemist in the last year of his PhD studies at Karolinska Institutet. He originally comes from Sevilla (Spain) and arrived in Stockholm 5 years ago. His background is mainly neuro oncology, since he focuses his research on understanding brain injury produced by radiotherapy, which is translated into cognitive problems afterwards.

🎾 He enjoys playing and watching tennis
🌱 Good gardener skills - he managed to grow tomatoes in Sweden
🍝 Pasta bolognese is his way of life

📸✨ Behind-the-Scenes of our Ebba Biotech Photoshoot!

Last Friday, the incredible Juliana Wolf Garcindo once again worked her magic to capture our company’s essence on camera.

🌟 Catch a sneak peek and meet our stunning team. Stay tuned for more glimpses of our behind-the-scenes moments!

🚀 Welcome 2024 with Ebba Biotech! 🌟

Happy New Year to our incredible community, both to our new and longstanding followers! 😊 As we step into 2024, why not kickstart by rediscovering our diverse product range? Explore the possibilities of enhancing your research with our cutting-edge tools - Amytracker, EbbaBiolight, and Carbotrace! 🧠🦠🌿

💡Wondering how to elevate your research? Check out our quick guide highlighting the opportunities our products can offer you today.

Get in touch and start your journey of discovery.🚀✨

We have recognized that optotracing is a novel concept that requires introduction and can sometimes be challenging. We strive towards making things as easy as possible for you. Therefore, we offer packages for "Microscopic Analysis" and "Spectroscopic Analysis" of your samples. We will provide you with protocols for labelling and data analysis as well as some professionally edited images to visualise your samples in a completely new light!