Protocol II: Measurement of cellulose content in a sample using Carbotrace 680

This protocol describes how to use Carbotrace 680 to determine cellulose content in a sample.

Solutions and Reagents:

Carbotrace 680 is provided as concentrated solution. The following common reagents are required (not supplied):

  • Phosphate buffered saline (PBS), pH 7.4
  • 96-well plate (round bottom)
  • Spectrophotometer

Assay Procedure:

  • Prepare a dilution series of your cellulose containing sample im PBS (e.g.: 1, 1:2, 1:5, 1:10, 1:100, 0)
  • Dilute Carbotrace 680 in PBS 1:500.
  • Add 50 µl of each cellulose containing dilution into a well of a 96-well plate.
  • Add 50 µl diluted Carbotrace 680 to each cellulose containing dilution and the blank control.
  • Measure emission using spectrophotometer settings (see 'Spectrophotometer Settings' below).

Spectrophotometer Settings:

  • Carbotrace 680: Excite at 540 nm and collect emission at 680 nm. Optional: Record an emission spectrum (560 - 800 nm) with 540 nm excitation.

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