Collection: Carbotrace
A sustainable solution for transforming food waste into high-value hydrogels
Researchers from the University of York focused on creating hydrogels from blackcurrant pomace, a residue from the food supply chain. They aimed to explore sustainable methods for producing valuable biopolymers like cellulose through microwave-assisted hydrothermal fractionation, thus reducing waste. The team first isolated pectin at various temperatures, then used a second microwave treatment to extract defibrillated cellulose from the remaining pomace residues. They assessed the cellulose’s ability to form hydrogels, a process enhanced through further bleaching steps. To visualise the cellulose and ensure effective extraction, the researchers utilised Carbotrace 480 binding to cellulose, allowing them to observe cellulose distribution via... Read more →
Carbotrace for differentiation between lignin and cellulose in renewable resources
A study by researchers from KTH explores Lupinus angustifolius (a type of lupin) as a sustainable source of lignocellulose materials. In order to reduce reliance on forest wood and utilise agricultural residues to produce microfibrillated cellulose (MFC), the team analysed the anatomical structure and chemical composition of the plant, classifying parts into stems, roots, seed pods, and seeds and developed a mild extraction process using alkaline treatment to create lignin-containing MFC (L-MFC) from non-edible residues. The researchers employed several methods to track changes during extraction: they used scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) to examine the plant's... Read more →
Carbotrace as a tool for the study of cellulose oxidation and paper aging
Since its invention, paper has skyrocketed technological advances as a key medium for recording thoughts and ideas. However, over time paper ages and oxidation leads to loss of strength and renders sheets of paper brittle. Therefore, the study of paper aging becomes relevant not only for the preservation of historical and artistic values, but also because competition from electronic media and increasing demands from customers force the pulp and paper industry to improve its competitive edge by optimizing production processes and paper quality. In a recent publication from the Departments of Physics and Chemistry as well as Biological, Chemical and... Read more →
Making greener cellulose hydrogel
The use of Microalgae as a renewable resource has attracted large interest due to their potential for use in the renewable energy, biopharmaceutical, and nutraceutical industries. Microalgae are renewable, sustainable, and economical sources of biofuels, bioactive medicinal products, and food ingredients. Recently, researchers around Professor Avtar Singh Matharu from the Green Chemistry Centre of Excellence in York, United Kingdom have developed a green process to produce cellulose hydrogel using native and spent microalgae. Cellulose extraction from microalgae without any pre-treatment is difficult, since the microalgal cell wall is very thick and rigid, but the researchers have developed a protocol for... Read more →
Carbohydrate content and elasticity of cell walls in elongating maize root
Investigation of plant growth and development is now more than ever an important field of study following the many challenges faced in agriculture. Accurate methods and computational models of plant cell- and organ change during growth are therefore essential to tackle problems like increased food demand in the wake of climate change. Researchers at Kazan Institute of Biochemistry and Biophysics aimed to determine if the mechanical properties of root tissues can control growth. Since growth rate and direction is determined by mechanical properties of plant cell walls, they used an approach based on atomic force microscopy (AFM) to study cell... Read more →
Breaking barriers in early detection of cancer
The ability to detect and isolate circulating tumor cells from blood could be a life-saving diagnostic tool for early detection of cancer and downstream analysis of tumor growth and metastasis. Researchers from the lab of Aman Russom at the Royal Institute of Technology (KTH) in Sweden have engineered a microfluidic device which captures circulating tumor cells from whole blood using antibodies immobilized on a cellulose nanofibril substrate. Using the device, circulating tumor cells were enriched and captured from whole blood. Importantly, 94.5 % of the captured tumor cells could be released from the device and isolated for downstream analysis. The... Read more →
Carbotrace 540 was used to determine purity of cellulose extracted from sea lettuce
The video clip shows the eco-friendly extraction of of pure cellulose from green macroalgae from the Swedish west coast. Researchers from the Royal Institute of Technology and Karolinska Institutet in Sweden reported about an environmentally friendly process to obtain pure cellulose nanofibrils from the green macroalgae ulva lactuca. Cellulose was extracted by sequential treatment with ethanol, hydrogen peroxide, sodium hydroxide, and hydrochloric acid. A mechanical homogenization process was used to disintegrate the cellulose-rich fraction into cellulose nanofibrils. Analysis of the monosugars reveal that the extracted cellulose contains mainly glucose, but also a fraction of xylose. Carbotrace 540 allowed the researchers... Read more →
Carbotrace-like molecules allow highly detailed visualization of cellulose in plant cells
If you always wondered why it is so difficult to switch to plants for producing renewable resources and how Carbotrace can help in the process, the video from Ben@SwedishMedicalNanoscienceCenter explains: "The complicated thing about plants and biofuels." A study from 2018 published in Nature Scientific Reports used a Carbotrace-like molecule to visualize the location and structure of cellulose in plant cells. Applying the molecule to thin slices of onion in a simple procedure resulted in highly fluorescent cell walls and allowed to obtain detailed 3D visualization of perforated sieve plates and plasmodesmata in onion cells. The fluorescence of chlorophyll containing... Read more →
Carbotrace 680 for carbohydrate anatomical mapping
The video clip below illustrates why our current model of Take, Make, Dispose is draining our planet of natural resources and why circular economy and renewable resources are the answer. Learn how Carbotrace can help to identify renewable resources in plant biomass and support circular economy in the food & beverage, pharmaceutical and chemical industry. In a research paper published in Cellulose, our structure-responsive optotracer molecule Carbotrace 680 was used to demonstrate the potential of optotracing for carbohydrate anatomical mapping and spectral imaging. As an example - to show the utility and ease of the new technology - Carbotrace 680... Read more →
Alina Schmidt about Carbotrace 680
Alina Schmidt about Carbotrace 680 "We used Carbotrace 680 to visualize cellulose in intact tissue of the macroalgae Ulva fenestrata and established 3D models of its architecture. This approach revealed new structural features, including a sandwich-like outermost layer in the blade and a remarkably thick median layer in the rhizoidal tissue. Carbotrace 680 was essential for identifying the cellulose distribution in situ, and enabled us to uncover tissue-specific differences that were previously overlooked." Alina Schmidt (PhD), PhD candidate, KTH, Stockholm, Sweden Fluorescence confocal microscopy visualising the lignocellulose anatomy in Carbotrace 680-stained Ulva fenestra (algae ) cross section. Fluorescent mapping with... Read more →Testimonial - Reina Tanaka
Reina Tanaka about Carbotrace & Ebba Biotech Research service: “Ebba Biotech helped us by performing analyses on Tunicate derived Cellulose nanocrystals and softwood TEMPO Oxidised Cellulose nanofibrils to determine the best Carbotrace molecule for our analysis. This encompassed both spectrophotometric testing and data analysis. This service was instrumental for us to continue tests for our own research.” Dr. Reina Tanaka from Forestry and Forest Products Research Institute, Forest Research and Management Organization, Tsukuba, Japan Testimonial given on October 6th, 2022 Read more →
Testimonial - Frederik Zitzmann
Prof. Avtar Matharu & Frederik Zitzmann (doctoral student) about Carbotrace 480: ”We used Carbotrace 480 as an analytical tool to map cellulose content and distribution in our defibrillated cellulose samples which we derived from microalgae. Due to the complex structure of the algal cell wall we had major difficulties in estimating the cellulose content in our samples and very clearly identify cellulose responses in the form of peaks of other types of analysis." ”Carbotrace 480 helped us in a major way as it specifically binds to cellulose and flags it up in green on a confocal laser microscope which gave... Read more →Testimonial - Saga Jakobsson
Saga Grånäs Jakobsson about Carbotrace 680: “I used Carbotrace 680 in my master thesis project where I investigated hemicelluloses and nanocellulose. I was able to detect hemicelluloses xyloglucan and galactomannan with Carbotrace 680. Also, I was able to use Carbotrace 680 to assess cellulose presence in commercially available samples and extracts and resolve fibrillar fine structures using Carbotrace 680 and confocal laser scanning microscope (CLSM).” Saga Grånäs Jakobsson from Department of Fibre- and Polymer Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), KTH Royal Institute of Technology, Stockholm, Sweden Testimonial given on September 8th, 2022 Read more →
Testimonial - Aristoteles Goes-Neto
Prof. Aristóteles Góes-Neto & Thairine Mendes-Pereira (PhD student) about Carbotrace 680: "We used Carbotrace 680 to label fungal cell wall chitin and spider exoskeleton chitin. More specifically, we studied the host-pathogen interaction of the pathogenic fungal genus Gibellula (Ascomycota) and the Pholcidae family of spiders. In our hands, Carbotrace 680 proved very useful. It stained all the chitin in the fungal cell wall and in the exoskeleton of spider legs. The fungal cell wall chitin was stained with an intense bright red and the spider exoskeleton in a darker red. Furthermore, the parts of the fungal cell wall with more... Read more →Testimonial - Ulrica Edlund
Prof. Ulrica Edlund about Carbotrace 540: "We were studying the biochemical composition of Ulva lactuca (a.k.a. Ulva fenestrata), which is still not fully known. Our hypothesis, and we wanted to prove, was the presence of cellulose and we knew that optotracing was probably the best method for analysis to distinguish cellulose from other glucans, but not which optotracing molecule would work the best. So we simply took a few of the optotracers we had already used plus the newest available variant [Carbotrace 540 from Ebba Biotech] and that one proved to work best of all! We saw a clear redshift... Read more →Testimonial - Ferdinand X. Choong
Dr. Ferdinand X. Choong about Carbotrace 680: "It really made carbohydrate detection so much easier! Thinking back on how to detect starch you had to use iodine solutions - that was kind of messy - and now Carbotrace 680 just works - simple and accurate." Dr. Ferdinand X. Choong is Assistant Professorat Karolinska Institutet and Team Leader at the Center for the Advancement of Integrated Medical and Engineering Sciences (AIMES) in Stockholm, Sweden. AIMES is a new center promoting interdisciplinary research, innovation, implementation and entrepreneurship and is funded by KTH Royal Institute of Technology and Karolinska Institutet. Testimonial given on... Read more →
Testimonial - Ulrica Edlund
Prof. Ulrica Edlund about Optotracing: Ulrica Edlund is Professor in Polymer technology at KTH Royal Institue of Technology and Vice Director at the Center for the Advancement of Integrated Medical and Engineering Sciences (AIMES) at Karolinska Institutet in Stockholm, Sweden. Optotracing in biorefinement of Canola Straw. Thin sections of canola straw were prepared for non-destructive optotracing analysis by fluorescence microscopy. Optotracing shows cellulose yellow and lignin blue-turquoise. The images were kindly provided by Prof. Ulrica Edlund. Testimonial given on April 29th, 2021 Read more →
Testimonial - Liudmila Kozlova
Dr. Liudmila Kozlova about Carbotrace 680: "We have used Carbotrace 680 to resolve the cellular structure of studied plants. It is possible due to the high content of cellulose in plant cell walls. Moreover, the fluorescence intensity of Carbotrace 680 can be used for the determination of cell wall thickness and, hence, as the normalization factor for quantification of labelling/staining by other cell wall-specific agents. Carbotrace 680 may be a better choice for cell wall thickness quantification than Calcofluor White in the case you are studying plant species that display high autofluorescence at UV irradiation (i.e. almost all Monocots). Carbotrace... Read more →
Testimonial - Tharagan Kumar
Tharagan Kumar about Carbotrace 680: Tharagan Kumar is doctoral student at KTH Royal Institute of Technology, Division of Nanobiotechnology located at SciLifeLab in Solna, Sweden. Tharagan sent us an image about how he was using Carbotrace 680 in his work. Optimization of an immobilization matrix made of cellulose nanofibrils in a microfluidic device. The images were kindly provided by Tharagan Kumar, KTH Royal Institue of Technology, Division of Nanobiotechnology. Testimonial given on April 29th, 2021 Read more →2026
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Płachno, B. J., Kapusta, M., Feldo, M., Stolarczyk, P., & Świątek, P. (2026). Immunocytochemical Analysis of the Wall Ingrowths and Cell Wall Microdomains in the Digestive Glands of Venus’ Flytrap. International Journal of Molecular Sciences. 27(3), 1193 https://doi.org/10.3390/IJMS27031193/S1
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Herting, G., Blomberg, E., Khort, A., Rogö, H., Palmi, K., Hammar, H., Richter-Dahlfors, A., & Odnevall, I. (2026). Mechanistic insights on surface adsorption of rice-based biomolecules on stainless steel 316L and its effects on corrosion and metal migration. Journal of Food Engineering, 413, 113018. https://doi.org/10.1016/J.JFOODENG.2026.113018
2025
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Bogdziewiez, L., Froeling, R., Schöppl, P., Juquel, J., Antoniadi, I., Skalický, V., Mathey, A., Fattaccioli, J., Sprakel, J., & Verger, S. (2025). The Q-Warg Pipeline: A Robust and Versatile Workflow for Quantitative Analysis of Protoplast Culture Conditions. Plant Direct, 9(7), e70090. https://doi.org/10.1002/pld3.70090
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Kapusta, M., Narajczyk, M., & Płachno, B. J. (2025). Arabinogalactan Proteins Mark the Generative Cell–Vegetative Cell Interface in Monocotyledonous Pollen Grains. Cells, Vol. 14, Page 1549, 14(19), 1549. https://doi.org/10.3390/cells14191549
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Płachno, B. J., Kapusta, M., Feldo, M., Stolarczyk, P., Małota, K., & Banaś, K. (2025). External Glands of Nepenthes Traps: Structure and Potential Function. International Journal of Molecular Sciences, 26(16), 7788. https://doi.org/10.3390/ijms26167788
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Costa, L., Carvalho, A. F., Fernandes, A. J. S., Campos, T., Dourado, N., Rodrigues, A. C., Sampaio, P., Costa, F. M., Dourado, F., & Gama, M. (2025). On the microstructural anisotropy and mechanical properties of bacterial nanocellulose obtained by static culture. International Journal of Biological Macromolecules, 330, 148114. https://doi.org/10.1016/j.ijbiomac.2025.148114
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Fatima, I., Wakade, G., & Daniell, H. (2025). Expression of mannanase and glucanases in lettuce chloroplasts and functional evaluation of enzyme cocktail against Candida albicans in oral cancer patient samples. Plant Biotechnology Journal, 23(7), 2689–2703. https://doi.org/10.1111/pbi.70046
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Mao, A., Gebhard, A. C., Ezazi, N. Z., Salhotra, A., Riazanova, A. v., Shanker, R., Wågberg, L., Nielsen, L. H., & Svagan, A. J. (2025). Plant cell–inspired colon-targeted cargo delivery systems with dual-triggered release mechanisms. Science Advances, 11(20), 2653. https://doi.org/10.1126/sciadv.adt2653
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Rueckel, M., & Pasquali, G. A. M. (2025). Spatial mapping of xylanase activity on maize meal. Animal Feed Science and Technology, 330, 116524. https://doi.org/10.1016/j.anifeedsci.2025.116524
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Schmidt, A. E. M., Richter-Dahlfors, A., & Edlund, U. (2025). Exploring the role of lignocellulose anatomy in the production and properties of lignin-containing microfibrillated cellulose from Lupinus angustifolius. Industrial Crops and Products, 237, 122262. https://doi.org/10.1016/j.indcrop.2025.122262
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Schmidt, A. E. M., Steinhagen, S., Nilsson, K. P. R., Edlund, U., & Richter-Dahlfors, A. (2025). Spatial in situ mapping of cellulose and other biopolymers reveals the 3D tissue architecture in the green algae Ulva fenestrata. International Journal of Biological Macromolecules, 320, 145632. https://doi.org/10.1016/j.ijbiomac.2025.145632
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Fatima, I., Wakade, G., Ahmad, N., & Daniell, H. (2025). Expression of endochitinase and exochitinase in lettuce chloroplasts increases plant biomass and kills fungal pathogen Candida albicans. Plant Biotechnology Journal, 23(5), 1437–1451. https://doi.org/10.1111/pbi.14596
2024
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Płachno, B. J., Kapusta, M., Feldo, M., & Swiatek, P. (2024) Homogalacturonans and Hemicelluloses in the External Glands of Utricularia dichotoma Traps. International Journal of Molecular Sciences. https://www.mdpi.com/1422-0067/25/23/13124
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Schmidt, A. E. M., Choong, F. X., Richter‐Dahlfors, A., & Edlund, U. (2024). Defibrillated Lignocellulose Recovery Guided by Plant Chemistry and Anatomy – A Pioneering Study with Lupinus angustifolius. Advanced Sustainable Systems. https://doi.org/10.1002/adsu.202300632
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Ferrara, V., Vetri, V., Pignataro, B., Chillura Martino, D. F., & Sancataldo, G. (2024). Phasor-FLIM analysis of cellulose paper ageing mechanism with carbotrace 680 dye. International Journal of Biological Macromolecules , 260, 129452. https://doi.org/10.1016/j.ijbiomac.2024.129452
2023
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Inthalaeng, N., Dugmore, T. I. J., & Matharu, A. S. (2023). Production of Hydrogels from Microwave-Assisted Hydrothermal Fractionation of Blackcurrant Pomace. Gels, 9(9). https://doi.org/10.3390/gels9090674
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Petrova, A., Ageeva, M., & Kozlova, L. (2023). Root growth of monocotyledons and dicotyledons is limited by different tissues. Plant Journal, 116(5), 1462–1476. https://doi.org/10.1111/tpj.16440
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Holzinger, A., Plag, N., Karsten, U., & Glaser, K. (2023). Terrestrial Trentepohlia sp. (Ulvophyceae) from alpine and coastal collection sites show strong desiccation tolerance and broad light and temperature adaptation. Protoplasma. https://doi.org/10.1007/s00709-023-01866-2
2022
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Zitzmann, F. L., Ward, E., & Matharu, A. S. (2022). Use of Carbotrace 480 as a Probe for Cellulose and Hydrogel Formation from Defibrillated Microalgae. Gels, 8(6), 383. https://doi.org/10.3390/gels8060383
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Petrova, A., Sibgatullina, G., Gorshkova, T., & Kozlova, L. (2022). Dynamics of cell wall polysaccharides during the elongation growth of rye primary roots. Planta, 255(5), 108. https://doi.org/10.1007/s00425-022-03887-2
2020
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Petrova, A., Gorshkova, T., & Kozlova, L. (2020). Gradients of cell wall nano-mechanical properties along and across elongating primary roots of maize. Journal of Experimental Botany. https://doi.org/10.1093/jxb/eraa561
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Kumar, T., Soares, R. R. G., Dholey, L. A., Ramachandraiah, H., Aval, N. A., Aljadi, Z., Pettersson, T., & Russom, A. (2020). Multi-layer assembly of cellulose nanofibrils in a microfluidic device for the selective capture and release of viable tumor cells from whole blood. Nanoscale, 42. https://doi.org/10.1039/d0nr05375a
2019
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Choong, F. X., Lantz, L., Shirani, H., Schulz, A., Nilsson, K. P. R., Edlund, U., & Richter-Dahlfors, A. (2019). Stereochemical identification of glucans by a donor–acceptor–donor conjugated pentamer enables multi-carbohydrate anatomical mapping in plant tissues. Cellulose, 26(7), 4253–4264. https://doi.org/10.1007/s10570-019-02381-5
Carbotrace fluorescence spectra
We named our Carbotrace molecules after their peak emission wavelength when they are bound to their target. That means, when Carbotrace is bound to a target, it will emit fluorescence at peak emission indicated by the number associated with its name.
To view the excitation and emission spectra, please select your Carbotrace below :
Protocol I: Anatomical mapping of cellulose in plant tissues using Carbotrace 680
This protocol describes a non-destructive method of anatomical mapping of cellulose structures in plant tissues. Solutions and Reagents: Carbotrace 680 is provided as concentrated solution. The following common reagents are required (not supplied): Phosphate buffered saline (PBS), pH 7.4 Assay Procedure: Prepare thin sections of plant tissue. Dilute Carbotrace 680 in PBS 1:1000. Apply diluted Carbotrace™ generously on the plant tissue. Use enough liquid (ca 0.5 ml) to prevent the sections from drying out during incubation. Incubate for 30 min. Wash 2 x 5 min in PBS. Mount sections for microscopic analysis. Fluorescence Microscopy: Carbotrace 680: Excite at 535 nm... Read more →Protocol II: Measurement of cellulose content in a sample using Carbotrace 680
This protocol describes how to use Carbotrace 680 to determine cellulose content in a sample. Solutions and Reagents: Carbotrace 680 is provided as concentrated solution. The following common reagents are required (not supplied): Phosphate buffered saline (PBS), pH 7.4 96-well plate (round bottom) Spectrophotometer Assay Procedure: Prepare a dilution series of your cellulose containing sample im PBS (e.g.: 1, 1:2, 1:5, 1:10, 1:100, 0) Dilute Carbotrace 680 in PBS 1:500. Add 50 µl of each cellulose containing dilution into a well of a 96-well plate. Add 50 µl diluted Carbotrace 680 to each cellulose containing dilution and the blank control.... Read more →
Multiplexing Carbotrace and Antibodies
When trying to understand and build in a world reliant on polymers, plant tissue is at the forefront of green chemistry with the abundance provided by polysaccharides. Techniques for the analysis of structures, morphology and localisation of different carbohydrates provides deep insight in a variety of fields, making in depth imaging and visualising crucial to basic research. When working with varied polysaccharides or other structural elements like proteins, researchers need to be able to distinguish components in complex environments such as within cells or in multicellular organisms reliably, which requires specificity. Currently one of the most commonly used techniques are... Read more →
Monitoring heat-induced swelling of Starch granules
As starch is a thermo-sensitive material that undergoes drastic morphological changes when heated, it is even possible to monitor starch reorganization during heat-induced swelling. When freshly extracted potato starch granules marked with Carbotrace 680 are heated from 20 °C to 90 °C and imaged with a confocal microscope, it becomes obvious that no change in granule size and morphology occurs until the granules are heated to 60 °C. At temperatures higher than 60 °C swelling is observed which can be identified by growing starch granules, but also by a visible change in the fluorescence color and intensity produced by Carbotrace... Read more →Carbotrace fluorescence spectra
We named our Carbotrace molecules after their peak emission wavelength when they are bound to their target. That means, when Carbotrace is bound to a target, it will emit fluorescence at peak emission indicated by the number associated with its name. To view the excitation and emission spectra, please select your Carbotrace below : Carbotrace 480 Carbotrace 520 Carbotrace 540 Carbotrace 630 Carbotrace 680 Excitation (blue lines) and emission (red lines) spectra of unbound Carbotrace (dotted lines) and Carbotrace bound to a target (solid lines). Read more →
Carbotrace 680 for monitoring starch
Detection of polysaccharides using Carbotrace 680 relies on regularly occurring units joined by glycosidic linkages. The specific type of glycosidic linkage and the occurrence of branches allow Carbotrace 680 to differentiate between different types of glucans. By means of its structure-responsive properties, Carbotrace 680 can differentiate between β(1-4) linked cellulose and α(1-4) configured amylose, but it can also detect differences between amylose and amylopectin, which contains α(1-6) linked branches. Since starches from different sources often differ by the length of glucose chains or the amylose/amylopectin ratio, and the protein and fat content of the storage organs, Carbotrace 680 can distinguish... Read more →Transfer cells in the glands of carnivorous plants
Ebba Biotech welcomes you to tune in to our webinar featuring Prof. Bartosz Plachno from Uniwersytet Jagielloński w Krakowie. He presents his research using Carbotrace to visualize transfer cells in the glands of carnivorous plants. Prof. Płachno is a biologist with a particular interest in carnivorous plants. His main research tool is microscopy, especially electron microscopy, and he is particularly interested in the functioning of carnivorous plant traps in the context of different glandular structures and surface specializations. Currently, he is focusing on cell walls in glandular cells (their composition and specialization). Read more →Incorporating Carbotrace as a problem solver in a biorefinery design process
Ebba Biotech welcomes you to tune in to our webinar featuring Dr. Frederik Zitzmann from the University of York. During his talk titled "Incorporating Carbotrace as a problem solver in a biorefinery design process.", Dr. Zitzmann will discuss how we can use novel technologies such as visualising with Carbotrace to revolutionise the world of sustainable materials. Dr. Frederik Zitzmann is a Green Chemistry researcher with particular interest in biorefinery design, industrial scale-up as well as client-focused research & development of products. He has an extensive background working on microalgae in collaboration with various start-ups and has designed microalgal biorefinery schemes... Read more →Cellulose in Cancer Diagnostics
Ebba Biotech welcomes you to listen to our newest webinar featuring Dr. Tharagan Kumar presenting his work from his time at KTH, the Royal Institute of Technology in Stockholm Sweden. During this talk titled “Cellulose in Cancer Diagnostics”, Dr. Kumar will be presenting his work breaking barriers in diagnostics using the Ebba Biotech Carbotrace molecules. Dr. Kumar comes from a background in biotechnology on the nanoscale leading him to seek the link between science and technology in medicine. With this, his studies from bachelors level all the way to doctoral studies focused heavily on how modern technology can improve lives... Read more →Spectral identification and recovery of polysaccharides from biorefining
Ebba Biotech welcomes you to listen to Professor Ulrica Edlund from the Royal Institute of Technology (KTH) in Stockholm and her revolutionary work with Carbotrace and nano-materials. The webinar titled "Selective method for spectral identification and recovery of polysaccharides from biorefining" explains how she uses Optotracers, like Carbotrace, in her research to discover and characterise renewable resources for biorefinement. As a leading researcher in her field, Prof. Edlund contributes to reach UN sustainability goals by paving the way for a greener future moving towards renewable materials and circular economy. Her passion for finding sustainable solutions to maximise the potential of... Read more →Optotracers - multifunctional fluorescent tracers
On the first of June 2021, Ferdinand Choong, Ebba Biotech's co-founder, and Assistant Professor at Karolinska Institutet and AIMES (Center for the Advancement of Integrated Medical and Engineering), presented his research using Ebba Biotech's optotracers at the digital event Lab & Diagnostics of the Future 2021, held by Life Science Sweden. At this event, Ferdinand spoke about Ebba Biotech's optotracers multifunctional tracer for disease research and diagnostics. He explains the technical concept in large and Ebba Biotech's three product series, Amytracker - used to detect amyloids and other protein aggregates, Ebba Biolight - used to detect bacteria and biofilm, Carbotrace... Read more →Carbotrace 540 helps to determine purity of plant raw materials
Researchers from the Royal Institute of Technology and Karolinska Institutet in Sweden reported about an environmentally friendly process to obtain pure cellulose nanofibrils from the green macroalgae ulva lactuca (Wahlström et al. (2020) Cellulose 27, 3707–3725). Analysis of the monosugars revealed that the extracted cellulose contains mainly glucose, but also a fraction of xylose. Carbotrace 540 allowed the researchers to analyze the carbohydrates in its polymeric state and was key to uncover that the macroalgae contain a mixture between highly pure and a xylose-glucose polysaccharide, such as xyloglucan. The video clip shows the eco-friendly extraction of of pure cellulose from... Read more →Carbotrace-like molecules for visualization of cellulose in plants
A research paper (Choong et al. (2018) Scientific Reports, 8, 3108) uses a Carbotrace-like molecule to visualize the location and structure of cellulose in plant cells. The video below explains why it is so difficult to switch to plants for producing renewable resources and how Carbotrace can help in the process. Read more →Carbotrace supports circular economy
In a research paper published in Cellulose (Choong et al. (2019) Cellulose, 26, 4253–4264), our structure-responsive optotracer molecule Carbotrace 680 was used to demonstrate the potential of optotracing for carbohydrate anatomical mapping and spectral imaging. The video clip below illustrates why our current model of Take, Make, Dispose is draining our planet of natural resources and why circular economy and renewable resources are the answer. Learn how Carbotrace can help to identify renewable resources in plant biomass and support circular economy in the food & beverage, pharmaceutical and chemical industry. Read more →-
Carbotrace 540
Regular price From €200,00 EURRegular priceSale price From €200,00 EUR
Carbotrace are optotracers for anatomical mapping of carbohydrate structures in plants and for non-destructive composition analysis of glucans in bio-based materials and biofuels.
The optotracing technology entails the use of structure-responsive fluorescent molecules (optotracers) which become fluorescent when binding to a target. The excitation and emission spectra of the optotracer molecule contain information on the structure of their binding partner and their environment. This spectral fingerprint can be used to identify biomolecules in various types of materials.
Carbotrace is available in five variants which bind to repetitive motifs in proteins and carbohydrates. It has been shown that Carbotrace 680 binds to homoglucans and heteroglucans with a limited degree of branching. By using the spectral fingerprint from the excitation- and emission spectra, it was shown that the optotracer can differentiate between different types of glycosidic bonds. Using Multi-laser/Multi-detector imaging, non-destructive composition analysis of biomass has been performed such as differentiation of cellulose cell walls and starch granules in fresh potato as well as cellulose and lignin in in various types of biomass. Using fluorescence lifetime imaging in combination with Carbotrace 680, the mechanism of paper ageing was analysed. Further, Carbotrace 680 was used to visualise cellulose content in terrestrial plants (maize root and ulvophyceae) as well as algae and to characterise a cellulose immobilisation matrix in a microfluidic device. Carbotrace 480 was used as a tool for cellulose visualisation and to optimise cellulose extraction from macroalgae.
Carbotrace variant work in a wide range of salt and pH conditions. When the pH is altered during the experiment, pH controls should be included. Carotrace can be used with fluorescence plate readers, fluorescence microscopes and confocal laser scanning microscopes and fluorescence life time imaging.
Store your Carbotracer product in the fridge and use the opened container within 12 months. Carbotrace is for research use only and is not for resale.