Carbotrace 540

Carbotrace 540

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Carbotrace 540 is our yellow optotracer for labeling repetitive motifs in proteins and carbohydrates. Generally, Carbotrace labeling is strongest with protein aggregates. Homoglucans like cellulose are labeled reliably, albeit with lower quantum yield. Heteroglucans with low degree of branching like starches are labeled and can be distingiushed by their spectral fingerpint. Generally labelling of hemicelluloses is weak or absent.

Specifically, Carbotrace 540 has been used to determine the purity of cellulose extracted from macroalgae by allowing analysis of extracted carbohydrates in their polymeric state. In addition, Carbotrace 540 has shown to bind to the hemicellulose xyloglucan. Contact us to learn more about applications for Carbotrace 540.

Table: Excitation- and emission wavelengths as well as recommended filter sets.
Exmax Emmax Recommended filter-sets
Carbotrace 540 480 nm 540 nm FITC, GFP, YFP

Carbotrace 540 is available as 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (See volumes and prices in the drop-down list below). The product should be diluted 1:1000 before use. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.

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