EbbaBiolight are optotracers for detection of bacteria & components of bacterial biofilm.

EbbaBiolight is available in five variants which label extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight variants have been used as follows: EbbaBiolight 480 labels extracellular matrix produced by P. aeruginosa but not C. albicans in a mircofluidic device during mono- and co-culture. EbbaBiolight 630 and EbbaBiolight 680 label peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. EbbaBiolight 680 labels curli in the extracellular matrix produced by Salmonella as well as β-glucans and chitins in yeast- and hypal forms of C. albicans. It has been used to track pellicle formation in B. cenocepacia and label extracellular matrix components in Pseudomonas and E. coli.

All EbbaBiolight variants are exceptionally photostable and fluorogenic. When bound to a target, the optotracers can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. All EbbaBiolight variants are intended for use in live-cultures. Fixation can lead to unspecific labelling of bacterial cells, regardless of them gram status. If fixation needs to be performed, we recommend to apply fixation after labelling.

EbbaBiolight variant work in a wide range of salt and pH conditions. When the pH is altered during the experiment, pH controls should be included. EbbaBiolight can be used with fluorescence plate readers, fluorescence microscopes and confocal laser scanning microscopes, fluorescence life time imaging, fluorescence cytometry, Total internal reflection fluorescence (TIRF) microscopy and Multiphoton microscopy.

Store your EbbaBiolight product in the fridge and use the opened container within 12 months. EbbaBiolight is for research use only and is not for resale.