This protocol describes how to monitor kinetics of Salmonella extracellular matrix (curli) production in liquid culture. The method described here is based on Choong et al. (2016) npj Biofilms and Microbiomes, 2, 16024 where isogenic... Read more
This protocol describes how to monitor kinetics of Salmonella extracellular matrix (curli) production in liquid culture. The method described here is based on Choong et al. (2016) npj Biofilms and Microbiomes, 2, 16024 where isogenic... Read more
This protocol describes how to use EbbaBiolight to visualise curli in biofilm forming on semi-solid agar in real-time. Curli is a functional amyloid produced by many Enterobactericeae involved in adhesion to surfaces, cell aggregation, and... Read more
This protocol describes how to grow Salmonella biofilm at an air-liquid interface using inclined glass coverslips and how to visualize Salmonella extracellular matrix component curli using EbbaBiolight. The method described here is based on Choong... Read more