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Protocol I: Staining of surface biofilm using EbbaBiolight 680

This protocol describes how to grow biofilm at the air-liquid interface using the inclined-coverslip method and how to detect the air-surface biofilm using EbbaBiolight 680. As the optotracer does not influence biofilm formation when used in recommended concentrations, it can be present in growing cultures. It is also possible to...
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Protocol II: Optotracing of biofilm in tissue sections using EbbaBiolight 680

This protocol describes how to stain for the biofilm markers curli and cellulose in tissue sections. EbbaBiolight can be used to stain tissue sections prepared by the most common techniques like paraffin embedding and freezing. We recommend fixation in ice-cold ethanol, but fixation in 4% paraformaldehyde works as well. We...
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Protocol III: Biofilm tracking in live cultures using EbbaBiolight 680

This protocol describes how to trace the formation of biofilm in bacterial cultures using EbbaBiolight 680 which binds to the biofilm markers curli and cellulose. As EbbaBiolight 680 does not influence biofilm formation when used in recommended concentrations, it can be present in growing cultures. We have tested EbbaBiolight 680...
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Protocol IV: Biofilm quantification in colony re-suspensions using EbbaBiolight 680

This protocol describes how to quantify the biofilm markers curli and cellulose in colony re-suspensions using EbbaBiolight 680. We have tested this procedure with Salmonella Enteritidis and Salmonella Typhimurium strains. For these strains we have not observed staining of intracellular or membrane components. Solutions and Reagents: EbbaBiolight 680 is provided...
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