Protocol I - Labelling of surface biofilm using EbbaBiolight
Protocol I - Labeling of surface biofilm using EbbaBiolight This protocol describes how to grow biofilm at the air-liquid interface using inclined glass coverslips and how to detect & visualize it using EbbaBiolight. When used in recommended concentrations in Salmonella biofilms, EbbaBiolight does not influence biofilm formation [1]. Therefore we...
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Protocol II: Optotracing of biofilm in tissue sections using EbbaBiolight 680
This protocol describes how to stain for the biofilm markers curli and cellulose in tissue sections. EbbaBiolight can be used to stain tissue sections prepared by the most common techniques like paraffin embedding and freezing. We recommend fixation in ice-cold ethanol, but fixation in 4% paraformaldehyde works as well. We...
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Protocol III: Biofilm tracking in live cultures using EbbaBiolight 680
This protocol describes how to trace the formation of biofilm in bacterial cultures using EbbaBiolight 680 which binds to the biofilm markers curli and cellulose. As EbbaBiolight 680 does not influence biofilm formation when used in recommended concentrations, it can be present in growing cultures. We have tested EbbaBiolight 680...
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Protocol IV: Biofilm quantification in colony re-suspensions using EbbaBiolight 680
This protocol describes how to quantify the biofilm markers curli and cellulose in colony re-suspensions using EbbaBiolight 680. We have tested this procedure with Salmonella Enteritidis and Salmonella Typhimurium strains. For these strains we have not observed staining of intracellular or membrane components. Solutions and Reagents: EbbaBiolight 680 is provided...
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Protocol V - Real-time monitoring of curli production in bacterial biofilms
Protocol V - Real-time monitoring of curli production in bacterial biofilms This protocol describes how to use EbbaBiolight to visualise curli in biofilm forming on semi-solid agar in real-time. Curli is a functional amyloid produced by many Enterobactericeae involved in adhesion to surfaces, cell aggregation, and biofilm formation. EbbaBiolight are...
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Protocol VI - Semi high-throughput analysis of curli in bacterial biofilms
Protocol VI - Semi high-throughput analysis of curli in bacterial biofilms This protocol describes how to use EbbaBiolight to visualise curli in biofilm forming on semi-solid agar in real-time. Curli is a functional amyloid produced by many Enterobactericeae involved in adhesion to surfaces, cell aggregation, and biofilm formation. EbbaBiolight are...
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