EbbaBiolight 630

EbbaBiolight 630

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EbbaBiolight 630 is our orange optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 630 labels peptidoglycan and lipoteichoic acids in the cell wall of certain gram positives like Staphylococci and E. faecalis. Contact us to learn more about EbbaBiolight applications.

As all our optotracers, EbbaBiolight 630 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 630 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below. 

Table: Excitation- and emission wavelengths as well as recommended filter sets.
Exmax Emmax Recommended filter-sets
EbbaBiolight 630 520 nm 630 nm PI, Cy3, TxRed, mCherry, Cy3.5


EbbaBiolight 630 is available as a 1 mg/ml solution in volumes ranging from 10 - 200 µL (see volumes and prices in the list below). The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.

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