This protocol describes a non-destructive method of anatomical mapping of cellulose structures in plant tissues. Solutions and Reagents: Carbotrace 680 is provided as concentrated solution. The following common reagents are required (not supplied): Phosphate buffered saline (PBS), pH 7.4 Assay Procedure: Prepare thin sections of plant tissue. Dilute Carbotrace 680...
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This protocol describes how to use Carbotrace 680 to determine cellulose content in a sample. Solutions and Reagents: Carbotrace 680 is provided as concentrated solution. The following common reagents are required (not supplied): Phosphate buffered saline (PBS), pH 7.4 96-well plate (round bottom) Spectrophotometer Assay Procedure: Prepare a dilution series...
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