EbbaBiolight 480 is our blue fluorescent optotracer for labeling extracellular and intracellular bacterial amyloids as well as certain glucans produced as part of the extracellular matrix. Specifically, EbbaBiolight 480 has been used to label extracellular matrix produced by P. aeruginosa but not C. albicans in a mircofluidic device during mono- and co-culture. Contact us to learn more about EbbaBiolight applications.
As all our optotracers, EbbaBiolight 480 is exceptionally photostable and fluorogenic. When bound to a target, EbbaBiolight 480 can be imaged using fluorescence microscopy and spectral information can be acquired using a fluorescence spectrophotometer. As spectral information can give hints of the nature of the target, we recommend to acquire excitation and emission spectra whenever possible. Use recommended filter sets as well as excitation- and emission wavelengths according to the table below. EbbaBiolight is intended for use in live-cultures and are not compatible with fixation. If fixation needs to be performed, we recommend to apply fixation after labelling.
|EbbaBiolight 480||420 nm||480 nm||DAPI|
EbbaBiolight 480 is available as a 1 mg/ml solution in volumes ranging from 10 - 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water. Please contact us for custom options.
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