Carbotrace Mix&Try

Carbotrace Mix&Try

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Carbotrace Mix&Try is our recommended option for starting out with using Carbotrace. It contains 10 µL of each variant. Testing each variant will allow you to determine which variant is best suited for your experiments and available instruments.

All Carbotrace variants label repetitive motifs in proteins and carbohydrates. Generally, Carbotrace labeling is strongest with protein aggregates. Homoglucans like cellulose are labeled reliably, albeit with lower quantum yield. Heteroglucans with low degree of branching like starches are labeled and can be distingiushed by their spectral fingerpint. Labelling of hemicelluloses is weak or absent.

Carbotrace 480 has been used to optimize a sustainable extraction method for cellulose hydrogels derived from microalgae and Carbotrace 680 has been used to image cellulose and starch components in plant cells and differentiate between those components using multi-laser/multi-detector analysis with confocal microscopy. Using a fluorescence spectrophotometer to acquire excitation and emission spectra, a unique spectral signature allows to differentiate between α(1-3), α(1-4), α(1-6), β(1-3), β(1-4) and β(1-6) linked glucans. As such, Carbotrace 680 labels cell wall components such as Cellulose, Chitin, Laminarin and the hemicellulose xyloglucan. Further, Carbotrace 680 has been used to determine relative carbohydrate content and elasticity of cell walls in plant material. Contact us to learn more about applications for Carbotrace.

Table: Excitation- and emission wavelengths as well as recommended filter sets.
Exmax Emmax Recommended filter-sets
Carbotrace 480 420 nm 480 nm DAPI
Carbotrace 520 460 nm 520 nm FITC, GFP
Carbotrace 540 480 nm 540 nm FITC, GFP, YFP
Carbotrace 630 520 nm 630 nm PI, Cy3, TxRed, mCherry, Cy3.5
Carbotrace 680 530 nm 680 nm PI, mCherry, Cy3.5

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