Ebba Biotech launches new optotracer series - EbbaBiolight

“My passion has always been to make our nanoscience and organic chemistry research available to a wider audience. Finally, after spending years on research and development, we are ready and able to launch the full wavelength range of our fluorescent tracer (optotracer) molecule for probing and detecting bacterial biofilm under its new name EbbaBiolight. says founder and chairman of Ebba Biotech AB, Professor Agneta Richter-Dahlfors.

Professor Dahlfors and her group at the Center for the Advancement of Integrated Medical and Engineering Sciences (AIMES) at Karolinska Institutet and Royal Institute for Technology (KTH) have coined the term optotracing in several highly ranked research publications.

The EbbaBiolight product series consists of five fluorescent tracer (optotracer) molecules EbbaBiolight 480, EbbaBiolight 520, EbbaBiolight 540, EbbaBiolight 630 and EbbaBiolight 680. All variants are available to order in two volumes: 50 µl and 100 µl, in vitro, 1 mg/ml solution. We also offer the EbbaBiolight Mix&Try kit which contains the complete product series (five optotracer molecules) in volume of 10 µl each, 1 mg/ml solution.

With exceptionally high signal-to-noise ratio and spectral properties that are clearly distinguishable from biological autofluorescence, we especially recommend EbbaBiolight 680 for tracking of biofilm in live cultures.” says professor Agneta Richter-Dahlfors.

EbbaBiolight 680 has formerly been known under the commercial name ECtracer 680.


Optotracing is an innovative visualization method developed by scientists for scientists ideal to use in fluorescence microscopy. With optotracing – using fluorescent tracer molecules – Ebba Biotech provides an innovative method for several expanding areas of research. The optotracing technology has been developed by a scientific expert team of researchers at Karolinska Institutet, Linköping University and the Royal Institute of Technology (KTH) in Sweden and is a result of many years of cutting-edge nanoscience and organic chemistry research.

Ebba Biotech has commercialized distribution and sales of fluorescent tracer molecules and today supply these in three different series:

  • EbbaBiolight – to probe and detect bacteria & biofilm.
  • Carbotrace - for anatomical mapping of plants to understand their composition
  • Amytracker - to search and detect amyloids and other protein aggregates

Using EbbaBiolight as optotracer

EbbaBiolight molecules allow direct visualization of bacteria or bacterial biofilm, without using antibodies or any toxic chemicals. The molecules become highly fluorescent when bound to a target, are non-toxic, and do not interfere with bacterial growth or biofilm formation at recommended concentrations so EbbaBiolight can be added to live cultures to track bacterial growth or biofilm formation in real-time using spectrophotometric analysis. Further, EbbaBiolight are photo- and thermostable and allow for easy handling in any application.

“We know from our own research that EbbaBiolight is excellent to probe and detect biofilm and believe EbbaBiolight can be very useful for microbiology laboratories, in hospitals as well as in industry with high demands to detect bacterial contamination and biofilm formation.” concludes professor Agneta Richter-Dahlfors.

Quick Facts

EbbaBiolight optotracer molecules are provided as 1 mg/ml solution in volumes of 50 µl and 100 µl and 10 µl in a Mix&Try kit and are:

  • Fast
  • Simple and easy to handle
  • Photo- and thermostable
  • Non-toxic
  • Accurate

About Ebba Biotech

Ebba Biotech AB was founded in 2016 by professor Agneta Richter-Dahlfors to enable commercial applications and more widespread use of fluorescent tracer (optotracers) molecules, the result of many years of cutting-edge nanoscience and organic chemistry research. Ebba Biotech headoffice is based at Medeon (Science Park for Life Science in Malmö, Sweden) and professor Agneta Richter-Dahlfors has remained majority owner and chairman of the board. From the subsidiary located in Karolinska Institutet Science Park in Solna, Ebba Biotech produces, sells and delivers vials of concentrated solution of the many variants of designed and purpose-built fluorescent tracer (optotracer) molecules to research laboratories and industry worldwide.

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