Live-cell imaging

All Amytracker variants cross the cell membrane of living cells without permeabilization. Due to their low background fluorescence and minimal interference with biological autofluorescence, we recommend Amytracker 630 or Amytracker 680 for live-cell imaging. As Amytracker do not bleach easily, they are excellently suited for repeated illumination during time-lapse imaging. If possible, use serum-free medium during incubation.

Solutions and Reagents:

• Amytracker - Aqueous or Amytracker - DMSO
• Imaging medium: Serum- and Phenol Red free cell culture medium

Assay Procedure:

• Dilute Amytracker in Imaging medium 1:1000.
• Incubate your cells in Imaging medium for 30 min under normal culture conditions.
• Image cells
• Replace Imaging medium with standard cell culture medium.

Readout:

• Perform imaging using the filter settings listed in the table below.