Carbotrace

Carbotrace 680 is our red optotracer for labeling repetitive motifs in proteins and carbohydrates. Generally, Carbotrace labeling is strongest with protein aggregates. Homoglucans like cellulose are labeled reliably, albeit with lower quantum yield. Heteroglucans with low degree of branching like starches are labeled and can be distingiushed by their spectral fingerpint. Generally labelling of hemicelluloses is weak or absent.

Specifically, Carbotrace 680 has been used to image cellulose and starch components in plant cells and differentiate between those components using multi-laser/multi-detector analysis with confocal microscopy. Using a fluorescence spectrophotometer to acquire excitation and emission spectra, a unique spectral signature allows to differentiate between α(1-3), α(1-4), α(1-6), β(1-3), β(1-4) and β(1-6) linked glucans. As such, Carbotrace 680 labels cell wall components such as Cellulose, Chitin, Laminarin and the hemicellulose xyloglucan. Further, Carbotrace 680 has been used to determine relative carbohydrate content and elasticity of cell walls in plant material. Contact us to learn more about applications for Carbotrace 680.

Carbotrace 680 is available in four different formulations (See volumes and prices in the drop-down list below): 

• Aqueous: 1 mg/ml solution in ultrapure water. The product should be diluted 1:1000 before use. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.
• DMSO: 1 mg/ml solution in DMSO to prevent solvent evaporation. The product should be diluted 1:1000 before use.
• Solid: 1 mg solid lyophilised in a sterile injection bottle.
• Drop&Shine: 5 ml ready-to-use product in mounting medium. Ideal for use in sections. Add a some Drop&Shine and mount your slide to detect biofilms within minutes.

Carbotrace Mix&Try is our recommended option for starting out with using Carbotrace. It contains 10 µL of each variant. Testing each variant will allow you to determine which variant is best suited for your experiments and available instruments.

All Carbotrace variants label repetitive motifs in proteins and carbohydrates. Generally, Carbotrace labeling is strongest with protein aggregates. Homoglucans like cellulose are labeled reliably, albeit with lower quantum yield. Heteroglucans with low degree of branching like starches are labeled and can be distingiushed by their spectral fingerpint. Labelling of hemicelluloses is weak or absent.

Carbotrace 480 has been used to optimize a sustainable extraction method for cellulose hydrogels derived from microalgae and Carbotrace 680 has been used to image cellulose and starch components in plant cells and differentiate between those components using multi-laser/multi-detector analysis with confocal microscopy. Using a fluorescence spectrophotometer to acquire excitation and emission spectra, a unique spectral signature allows to differentiate between α(1-3), α(1-4), α(1-6), β(1-3), β(1-4) and β(1-6) linked glucans. As such, Carbotrace 680 labels cell wall components such as Cellulose, Chitin, Laminarin and the hemicellulose xyloglucan. Further, Carbotrace 680 has been used to determine relative carbohydrate content and elasticity of cell walls in plant material. Contact us to learn more about applications for Carbotrace.

Carbotrace 520 is our green optotracer for labeling repetitive motifs in proteins and carbohydrates. Generally, Carbotrace labeling is strongest with protein aggregates. Homoglucans like cellulose are labeled reliably, albeit with lower quantum yield. Heteroglucans with low degree of branching like starches are labeled and can be distingiushed by their spectral fingerpint. Generally labelling of hemicelluloses is weak or absent. Contact us to learn more about applications for Carbotrace 520.

Carbotrace 520 is available as 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (See volumes and prices in the drop-down list below). The product should be diluted 1:1000 before use. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.

Carbotrace 630 is our orange optotracer for labeling repetitive motifs in proteins and carbohydrates. Generally, Carbotrace labeling is strongest with protein aggregates. Homoglucans like cellulose are labeled reliably, albeit with lower quantum yield. Heteroglucans with low degree of branching like starches are labeled and can be distingiushed by their spectral fingerpint. Generally labelling of hemicelluloses is weak or absent. Contact us to learn more about applications for Carbotrace 630.

Carbotrace 630 is available as 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (See volumes and prices in the drop-down list below). The product should be diluted 1:1000 before use. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.

Carbotrace 540 is our yellow optotracer for labeling repetitive motifs in proteins and carbohydrates. Generally, Carbotrace labeling is strongest with protein aggregates. Homoglucans like cellulose are labeled reliably, albeit with lower quantum yield. Heteroglucans with low degree of branching like starches are labeled and can be distingiushed by their spectral fingerpint. Generally labelling of hemicelluloses is weak or absent.

Specifically, Carbotrace 540 has been used to determine the purity of cellulose extracted from macroalgae by allowing analysis of extracted carbohydrates in their polymeric state. In addition, Carbotrace 540 has shown to bind to the hemicellulose xyloglucan. Contact us to learn more about applications for Carbotrace 540.

Carbotrace 540 is available as 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (See volumes and prices in the drop-down list below). The product should be diluted 1:1000 before use. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.

Carbotrace 480 is our blue optotracer for labeling repetitive motifs in proteins and carbohydrates. Generally, Carbotrace labeling is strongest with protein aggregates. Homoglucans like cellulose are labeled reliably, albeit with lower quantum yield. Heteroglucans with low degree of branching like starches are labeled and can be distingiushed by their spectral fingerpint. Generally labelling of hemicelluloses is weak or absent.

Specifically, Carbotrace 480 has been used to optimize a sustainable extraction method for cellulose hydrogels derived from microalgae. Contact us to learn more about applications for Carbotrace 480.

Carbotrace 480 is available as 1 mg/ml solution in ultrapure water (Aqueous) with volumes ranging from 10 - 200 µL (See volumes and prices in the drop-down list below). The product should be diluted 1:1000 before use. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly.