Carbotrace 680 is our red fluorescent optotracer molecule for labeling glucans and some hemicelluloses. Generally, labeling is strongest with homopolymers compared to highly branched polysaccharides. Optotracers are structure-responsive molecules which display a unique spectral signature when binding to a target structure. Therefore, depending on the sample composition, it is possible to determine the purity of cellulose in the sample.
Specifically, Carbotrace 680 has been used to image cellulose and starch components in plant cells and differentiate between those components using multi-laser/multi-detector analysis with confocal microscopy. Using a fluorescence spectrophotometer to acquire excitation and emission spectra, a unique spectral signature allows to differentiate between α(1-3), α(1-4), α(1-6), β(1-3), β(1-4) and β(1-6) linked glucans. As such, it labels cell wall components such as Cellulose, Chitin, Laminarin and the hemicellulose xyloglucan. Further, Carbotrace 680 has been used to determine relative carbohydrate content and elasticity of cell walls in plant material. Contact us to learn more about applications for Carbotrace 680.
|Carbotrace 680||530 nm||680 nm||PI, mCherry, Cy3.5|
Carbotrace 680 is available as a 1 mg/ml solution in volumes ranging from 10 - 200 µL (see volumes and prices in the list below). As our standard solvent, we use ultrapure water (MilliQ). We also provide Carbotrace 680 dissolved in DMSO Please contact us for custom options.
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