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Use Amytracker™ to investigate amyloid formation

Amytracker™ Fibrillation

Use Amytracker™ to investigate amyloid formation

The kinetics of amyloid formation from conformational conversion of a peptide or protein into its fibrillar form (amyloid) is studied using fibrillation assays using a spectrophotometer. This technique requires extremely low background fluorescence of the unbound probe and Thioflavin T has been widely used for this reason. The kinetic profile typically includes a lag phase that is followed by a rapid exponential growth phase and a plateau phase. As shown...

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Superresolution microscopy

Superresolution

Superresolution microscopy

Super-resolution microscopy is becoming an important tool to study biological structures. As super-resolution techniques like STED overcome the physical diffraction limit of light, new microscopes with ever-decreasing resolution limits are being developed. Using these exciting techniques, the constraints are now imposed by the probes used for labelling. With STED microscopy, reaching a resolution of 20–40 nm, antibodies are no longer suitable as labelling probes sinceconjugated fluorophores will seem to be...

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Staining of Amyloids in Tissue Sections

Amytracker™

Staining of Amyloids in Tissue Sections

Amyloids are causative agents in many diseases. More than 30 different precursor proteins and peptides have been reported to form disease-associated amyloids in vivo and the role of pre-fibrillar amyloid species in amyloid toxicity is currently being investigated. As amyloid formation in vivo is slowly progressing, it often takes many years from on-set amyloid formation to the emergence of symptoms. Accurate identification of amyloid deposits and pre-fibrillar species is therefore a necessary...

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