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Live Cell Imaging

Live Cell Imaging

As functional aspects of amyloids as well as the dynamic processes involving amyloid formation and amyloid toxicity are of growing interest many researchers are interested to study these processes in living cells. Non-invasive techniques like fluorescence microsopy have been perfected in recent years for the study of living cells. Unfortunately, few non-toxic fluoresecent dyes with high affinity to amyloids exist. Amytracker have been shown to be non-toxic fluorescent probes binding...
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Amyloids - The Dark Matter of Biology

Amyloids - The Dark Matter of Biology

Under some conditions, peptides or proteins may convert from their soluble forms into unsoluble highly ordered fibrillar aggregates. These aggregates may cause disease through various mechanisms. Prominent examples of aggregating peptides related to neurodegenerative diseases are Amyloid β peptides which play an important role in Alzheimer's disease and aggregating α-synuclein which is found in Lewy bodies that are a hallmark of Parkinson's disease and some forms of dementia. Multiple variants...
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Amytracker for amyloid staining

Amytracker for amyloid staining

Amyloid detection has been notoriously difficult since current methods are either laborious, toxic and/or tend to detect mature fibrils but not protofibrils or premature aggregates. Amytracker are fluorescent tracer molecules binding to amyloids with high sensitivity. Amytracker have been shown to bind to prefibrillar states of amyloids and might therefore help to detect and investigate earlier prepathological states of amyloid diseases. As Amytracker are fluorescent only when bound to their...
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Use Amytracker to investigate amyloid formation

Use Amytracker to investigate amyloid formation

The kinetics of amyloid formation from conformational conversion of a peptide or protein into its fibrillar form (amyloid) is studied using fibrillation assays using a spectrophotometer. This technique requires extremely low background fluorescence of the unbound probe and Thioflavin T has been widely used for this reason. The kinetic profile typically includes a lag phase that is followed by a rapid exponential growth phase and a plateau phase. As shown...
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Staining of Amyloids in Tissue Sections

Staining of Amyloids in Tissue Sections

Amyloids are causative agents in many diseases. More than 30 different precursor proteins and peptides have been reported to form disease-associated amyloids in vivo and the role of pre-fibrillar amyloid species in amyloid toxicity is currently being investigated. As amyloid formation in vivo is slowly progressing, it often takes many years from on-set amyloid formation to the emergence of symptoms. Accurate identification of amyloid deposits and pre-fibrillar species is therefore...
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In vivo amyloid staining

In vivo amyloid staining

Intravital imaging is allowing researchers to capture images of biological processes in live animals. It has become an advanced tool to study the progression of Alzheimer's disease in transgenic mice. In vivo imaging using two-photon microscopy is an advantageous technique for observing tissues and organs at high resolution. Amytracker™ are suitable for in vivo studies since our probes are non-toxic, cross the blood-brain barrier and stain fibrous deposits in animal...
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