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How and Where is Amytracker™ binding?

Amytracker™

All Amytracker™ molecules are designed to interact with the same binding site on the amyloid oligomer. Competition assays have shown that Amytracker™ competes for the congo red binding site but show much higher affinity. In essence the binding cavity and binding mode of Amytracker™ is is dictated by a groove lined with repetitive positive charged side-chains. The detection of prefibrillar species appears dependent on 8 repetitive β sheets, composed of...

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What kind of fixation works best for Amytracker™ stainings?

Amytracker™

Generally, we recommend light fixation using ice-cold ethanol or acetone. This is because formalin-fixation has been shown to reduce the ability to stain inclusion bodies. Otherwise there shouldn't be any issues. You can use paraffin sections or cryosections. Note that epitope exposure and antigen retrieval is not needed when applying Amytracker™ to paraffin embedded sections. Read More: Åslund et. al. 2009, ACS Chemical Biology, 4(8), 673-684.

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How does Amytracker™ show the difference between Aβ plaques and τ tangles?

Amytracker™

Amytracker™ bind to disease associated protein aggregates and adopt a complementary geometry unique to the interaction with the target molecule. The conformational change when binding to a specific target results in a red-shift in emission wavelengths. This occurs with different extents between Aβ plaques and τ tangles. Thus, the different aggregated species, Aβ plaques and τ tangles, display distinct emission spectra when stained with Amytracker™. Read More: Klingstedt et. al....

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Can I use Amytracker™ for staining of protein aggregates live animals?

Amytracker™

Presently we supply Amytracker™ optimized for staining of protein aggregates in tissue sections (histology) or cells. Nevertheless, Amytracker™ have been shown to be non-toxic, non-immunogenic and readily pass the blood-brain-barrier. Already 6 min after i.v. injection, cerebral amyloid deposits can be imaged in mouse models of cerebral amyloid diseases using intravital multiphoton microscopy Amytracker™ for intravital staining of protein aggregates will soon be available through our webshop. If you are...

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Can I use Amytracker™ for staining of protein aggregates in life cell cultures?

Amytracker™

Amytracker™ have been shown to readily pass membranes of living cells such as dorsal root ganglion cells and human pancreatic islet cells and bind intracellular protein aggregates. No cytotoxic effects have been observed even with high concentrations of Amytracker™. For use in living cells, we recommend to dilute Amytracker™ 1:500 in cell culture medium and incubate cells for 30 min under cell culture conditions. We developed the deep red Amytracker™680...

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