Amytracker have been shown to readily pass membranes of living cells such as dorsal root ganglion cells and human pancreatic islet cells and bind intracellular protein aggregates. No cytotoxic effects have been observed even with high concentrations of Amytracker. For use in living cells, we recommend to dilute Amytracker 1:500 in cell culture medium and incubate cells for 30 min under cell culture conditions. We developed the deep red Amytracker 680 specifically for in vivo applications to avoid interference with autofluorescence common in live cells and tissues. Amytracker 540 is our green/yellow fluorescent tracer molecule specifically designed for superior uptake into cells and tissues.