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How sensitive is Amytracker™ when compared to Congo Red?

Amytracker™

Amytracker™ fluorescence is one magnitude brighter than Congo Red. The affinity of Amytracker™ is in the nM range and thus, Amytracker™ is typically used at several fold lower concentrations. In a comparative study using human amyloidosis tissue, Amytracker™ detected amyloid deposits in 15% of Congo Red negative samples. While the pathological relevance of Congo Red negative deposits is currently unclear, Amytracker™ staining might give an indication of earlier states of...

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Is Amytracker™ staining dependent on the tissue type or species?

Amytracker™

It is likely that Amytracker™ works on all kinds of tissues and species. Amytracker™ targets and detects the physical topography of the tertiary- and quaternary structure of mature and pre-fibrillar amyloid deposits. As such it is applicable to a wide range of animal models and different Amytracker™ molecules have been applied on tissue sections from humans, mice, cows, sheep, deer and Drosophila.

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Does Amytracker™ work to identify amyloids in different forms of human amyloid diseases?

Amytracker™

Amytracker™ have been shown to bind with high affinity to aggregates composed of transthyretin (TTR) in Drosophila models of transthyretin amyloidosis (ATTR), as well as in human tissues. Amytracker™ has been used to detect aggregates in following forms of human amyloid diseases: AA amyloidosis associated with accumulation of serum amyloid A (SAA) protein, isolated atrial amyloidosis associated with accumulation of atrial natriuretic factor, various forms of amyloid light chain (AL)...

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How should I dilute the supplied molecules?

Amytracker™ ECtracer™ Support

We supply optical tracer molecules with high affinity. For staining of cryo- or paraffin sections, diluting the supplied solutions 1:1000 should be sufficient. If you want to increase the intensity, you might increase the concentration and use 1:500 dilution instead. For life cell staining, we usually recommend to dilute our products 1:500. For fibrillation assays and spectroscopic application you should titrate the supplied optical tracer molecules, so you work with...

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How is Amytracker™ better than Thioflavin?

Amytracker™

When Amytracker™ are not bound to a target, they exhibit an extremely low background fluorescence. Amytracker™ have also ben shown to neither accelerate nor inhibit amyloid formation when used in recommended (substochoimetric) concentrations. Therefore, Amytracker™ are suitable for fibrillation assays and spectrophotometric detection. Amytracker™ have been shown to identify pre-fibrillar non-thioflavinophilic assemblies during in vitro fibrillation of Aβ peptides, insulin, lysozyme and prion protein with significantly reduced lag-phase compared to...

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