Dr. Andrea Sass about EbbaBiolight 680:
"In the Laboratory of Pharmaceutical Microbiology, Ghent University, we used EbbaBiolight 680 for visualizing extracellular matrix in pellicles formed by Pseudomonas aeruginosa. We found that the product labels pellicle matrix of P. aeruginosa specifically, bacterial cells were not labelled. The method revealed structural differences between pellicles of different strains and mutants, and gave us valuable insights into the pellicle structure. We were particularly impressed by how easy the optotracer solution is to use, just dilute it in phosphate buffered saline, or in medium. The cells can be grown in the presence of the optotracer, which means they can be microscopically observed without an additional staining step which could disrupt the structures. If necessary, post-labelling of pellicles was performed and also worked very well for us. There was no need for incubation time, the pellicles were labelled immediately. The fluorescence emitted by the optotracers is very stable, we did not observe any photobleaching during fluorescence microscopy. Overall, EbbaBiolight is a very useful additional tool for investigating the extracellular matrix of P. aeruginosa."
Dr. Andrea Sass, Laboratory of Pharmaceutical Microbiology, Ghent University, Belgium
Labeling of Pseudomonas aeruginosa PAO1 and PAO14 biofilm with EbbaBioligth 680. The overlay with brightfield images show that the pellicle biofilm is labeled with EbbaBiolight 680 (red) but not single bacteria. Images were kindly provided from Dr. Andrea Sass and Prof. Tom Coenye, Laboratory of Pharmaceutical Microbiology, Ghent University