Dr. Liudmila Kozlova about Carbotrace 680:
"We have used Carbotrace 680 to resolve the cellular structure of studied plants. It is possible due to the high content of cellulose in plant cell walls. Moreover, the fluorescence intensity of Carbotrace 680 can be used for the determination of cell wall thickness and, hence, as the normalization factor for quantification of labelling/staining by other cell wall-specific agents. Carbotrace 680 may be a better choice for cell wall thickness quantification than Calcofluor White in the case you are studying plant species that display high autofluorescence at UV irradiation (i.e. almost all Monocots). Carbotrace 680 also may help if for some reasons you cannot use UV excitation and/or blue light detection. Our experience with that molecule was just great. We definitely will use it again."
Dr. Liudmila Kozlova, Kazan Scientific Center of the Russian Academy of Sciences, Kazan Institute of Biochemistry and Biophysics, Kazan, Russia
Fluorescence micrographs of maize root cross-sections stained with Carbotrace 680. The images were kindly provided by Dr. Anna Petrova, Kazan Scientific Center of the Russian Academy of Sciences, Kazan Institute of Biochemistry and Biophysics, Kazan, Russia.