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Testimonial about Carbotrace 680 - Aristóteles Góes-Neto & Thairine Mendes-Pereira

Prof. Aristóteles Góes-Neto & Thairine Mendes-Pereira (PhD student) about Carbotrace 680:

"We used Carbotrace 680 to label fungal cell wall chitin and spider exoskeleton chitin. More specifically, we studied the host-pathogen interaction of the pathogenic fungal genus Gibellula (Ascomycota) and the Pholcidae family of spiders.

In our hands, Carbotrace 680 proved very useful. It stained all the chitin in the fungal cell wall and in the exoskeleton of spider legs. The fungal cell wall chitin was stained with an intense bright red and the spider exoskeleton in a darker red. Furthermore, the parts of the fungal cell wall with more chitin - for instance, the septa between cell compartments - were stained more strongly.

We will pursue more detailed analysis using confocal microscopy to further our investigation into this biological system."

Prof. Aristóteles Góes-Neto & Thairine Mendes-Pereira (PhD student)from Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

Carbotrace 680 was used to visualise chitin in the cell wall of the pathogenic fungal species Gibellula pulchra parasitizing spider legs of the Pholcidae family using a Leica DFC7000T epifluorescence microscope: A) Exoskeleton of spider legs B) Conidiophores C) Mycelial hyphae. The images were kindly provided by Prof. Aristóteles Góes-Neto & Thairine Mendes-Pereira (PhD student)from Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

Testimonial given on October 19th, 2021


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